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2pi5

From Proteopedia

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{{Seed}}
 
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[[Image:2pi5.png|left|200px]]
 
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<!--
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==T7 RNA polymerase complexed with a phi10 promoter==
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The line below this paragraph, containing "STRUCTURE_2pi5", creates the "Structure Box" on the page.
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<StructureSection load='2pi5' size='340' side='right'caption='[[2pi5]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2pi5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_phage_T7 Escherichia phage T7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PI5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PI5 FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pi5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pi5 OCA], [https://pdbe.org/2pi5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pi5 RCSB], [https://www.ebi.ac.uk/pdbsum/2pi5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pi5 ProSAT]</span></td></tr>
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{{STRUCTURE_2pi5| PDB=2pi5 | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/RPOL_BPT7 RPOL_BPT7] DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Responsible for the transcription of the late genes of T7. It is rifampicin-resistant. It recognizes a specific promoter sequence, unwinds the double-stranded RNA to expose the coding strand for templating, initiates transcription preferentially with a purine.
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/2pi5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pi5 ConSurf].
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<div style="clear:both"></div>
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===T7 RNA polymerase complexed with a phi10 promoter===
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==See Also==
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*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
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__TOC__
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<!--
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</StructureSection>
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The line below this paragraph, {{ABSTRACT_PUBMED_17512007}}, adds the Publication Abstract to the page
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[[Category: Escherichia phage T7]]
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(as it appears on PubMed at http://www.pubmed.gov), where 17512007 is the PubMed ID number.
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[[Category: Large Structures]]
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[[Category: Kennedy WP]]
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{{ABSTRACT_PUBMED_17512007}}
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[[Category: Momand JR]]
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[[Category: Yin YW]]
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==About this Structure==
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2PI5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_t7 Enterobacteria phage t7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PI5 OCA].
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==Reference==
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Mechanism for de novo RNA synthesis and initiating nucleotide specificity by t7 RNA polymerase., Kennedy WP, Momand JR, Yin YW, J Mol Biol. 2007 Jul 6;370(2):256-68. Epub 2007 Mar 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17512007 17512007]
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[[Category: DNA-directed RNA polymerase]]
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[[Category: Enterobacteria phage t7]]
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[[Category: Single protein]]
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[[Category: Kennedy, W P.]]
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[[Category: Momand, J R.]]
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[[Category: Yin, Y W.]]
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[[Category: Initiating nucleoite]]
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[[Category: T7 rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:29:50 2008''
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Current revision

T7 RNA polymerase complexed with a phi10 promoter

PDB ID 2pi5

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