This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2pia

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2pia" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pia, resolution 2.0&Aring;" /> '''PHTHALATE DIOXYGENASE...)
Current revision (09:09, 21 February 2024) (edit) (undo)
 
(15 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2pia.gif|left|200px]]<br /><applet load="2pia" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2pia, resolution 2.0&Aring;" />
 
-
'''PHTHALATE DIOXYGENASE REDUCTASE: A MODULAR STRUCTURE FOR ELECTRON TRANSFER FROM PYRIDINE NUCLEOTIDES TO [2FE-2S]'''<br />
 
-
==Overview==
+
==PHTHALATE DIOXYGENASE REDUCTASE: A MODULAR STRUCTURE FOR ELECTRON TRANSFER FROM PYRIDINE NUCLEOTIDES TO [2FE-2S]==
-
Phthalate dioxygenase reductase (PDR) is a prototypical iron-sulfur, flavoprotein (36 kilodaltons) that utilizes flavin mononucleotide (FMN) to, mediate electron transfer from the two-electron donor, reduced, nicotinamide adenine nucleotide (NADH), to the one-electron acceptor, [2Fe-2S]. The crystal structure of oxidized PDR from Pseudomonas cepacia, has been analyzed at 2.0 angstrom resolution resolution; reduced PDR and, pyridine nucleotide complexes have been analyzed at 2.7 angstrom, resolution. NADH, FMN, and the [2Fe-2S] cluster, bound to distinct, domains, are brought together near a central cleft in the molecule, with, only 4.9 angstroms separating the flavin 8-methyl and a cysteine sulfur, ligated to iron. The domains that bind FMN and [2Fe-2S] are packed so that, the flavin ring and the plane of the [2Fe-2S] core are approximately, perpendicular. The [2Fe-2S] group is bound by four cysteines in a site, resembling that in plant ferredoxins, but its redox potential (-174, millivolts at pH 7.0) is much higher than the potentials of plant, ferredoxins. Structural and sequence similarities assign PDR to a distinct, family of flavoprotein reductases, all related to ferredoxin, NADP(+)-reductase.
+
<StructureSection load='2pia' size='340' side='right'caption='[[2pia]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
 
+
== Structural highlights ==
-
==About this Structure==
+
<table><tr><td colspan='2'>[[2pia]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PIA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PIA FirstGlance]. <br>
-
2PIA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Burkholderia_cepacia Burkholderia cepacia] with FMN and FES as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2PIA OCA].
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
 
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-
==Reference==
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pia FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pia OCA], [https://pdbe.org/2pia PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pia RCSB], [https://www.ebi.ac.uk/pdbsum/2pia PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pia ProSAT]</span></td></tr>
-
Phthalate dioxygenase reductase: a modular structure for electron transfer from pyridine nucleotides to [2Fe-2S]., Correll CC, Batie CJ, Ballou DP, Ludwig ML, Science. 1992 Dec 4;258(5088):1604-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1280857 1280857]
+
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/PDR_BURCE PDR_BURCE] Component of the electron transfer chain involved in pyridine nucleotide-dependent dihydroxylation of phthalate. Utilizes FMN to mediate electron transfer from the two-electron donor, NADH, to the one-electron acceptor, (2Fe-2S).
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pi/2pia_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pia ConSurf].
 +
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Burkholderia cepacia]]
[[Category: Burkholderia cepacia]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Ballou, D.P.]]
+
[[Category: Ballou DP]]
-
[[Category: Batie, C.J.]]
+
[[Category: Batie CJ]]
-
[[Category: Correll, C.C.]]
+
[[Category: Correll CC]]
-
[[Category: Ludwig, M.L.]]
+
[[Category: Ludwig ML]]
-
[[Category: FES]]
+
-
[[Category: FMN]]
+
-
[[Category: reductase]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:32:52 2007''
+

Current revision

PHTHALATE DIOXYGENASE REDUCTASE: A MODULAR STRUCTURE FOR ELECTRON TRANSFER FROM PYRIDINE NUCLEOTIDES TO [2FE-2S]

PDB ID 2pia

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2pia"
Personal tools