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2pr5

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(New page: 200px<br /><applet load="2pr5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2pr5, resolution 1.450&Aring;" /> '''Structural Basis fo...)
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[[Image:2pr5.jpg|left|200px]]<br /><applet load="2pr5" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2pr5, resolution 1.450&Aring;" />
 
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'''Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Dark Structure)'''<br />
 
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==Overview==
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==Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Dark Structure)==
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The photosensor YtvA binds flavin mononucleotide and regulates the general, stress reaction in Bacillus subtilis in response to blue light, illumination. It belongs to the family of light-oxygen-voltage (LOV), proteins that were first described in plant phototropins and form a, subgroup of the Per-Arnt-Sim (PAS) superfamily. Here, we report the, three-dimensional structure of the LOV domain of YtvA in its dark and, light states. The protein assumes the global fold common to all PAS, domains and dimerizes via a hydrophobic interface. Directly C-terminal to, the core of the LOV domain, an alpha-helix extends into the solvent. Light, absorption causes formation of a covalent bond between a conserved, cysteine residue and atom C(4a) of the FMN ring, which triggers, rearrangements throughout the LOV domain. Concomitantly, in the dark and, light structures, the two subunits of the dimeric protein rotate relative, to each other by 5 degrees . This small quaternary structural change is, presumably a component of the mechanism by which the activity of YtvA is, regulated in response to light. In terms of both structure and signaling, mechanism, YtvA differs from plant phototropins and more closely resembles, prokaryotic heme-binding PAS domains.
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<StructureSection load='2pr5' size='340' side='right'caption='[[2pr5]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2pr5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. The March 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Phototropin'' by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_3 10.2210/rcsb_pdb/mom_2015_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PR5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PR5 FirstGlance]. <br>
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2PR5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] with NA, FMN and ACY as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2PR5 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pr5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pr5 OCA], [https://pdbe.org/2pr5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pr5 RCSB], [https://www.ebi.ac.uk/pdbsum/2pr5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pr5 ProSAT]</span></td></tr>
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Structural Basis for Light-dependent Signaling in the Dimeric LOV Domain of the Photosensor YtvA., Moglich A, Moffat K, J Mol Biol. 2007 Oct 12;373(1):112-26. Epub 2007 Aug 2. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17764689 17764689]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/PHOT_BACSU PHOT_BACSU] Exhibits the same spectroscopical features and blue-light induced photochemistry as plants phototropins, with the reversible formation of a blue-shifted photoproduct, assigned to an FMN-cysteine thiol adduct. Although it is a positive regulator in the activation of the environmental signaling branch of the general stress transcription factor sigma-B, its precise role is undetermined.<ref>PMID:11157946</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/pr/2pr5_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2pr5 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Moffat, K.]]
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[[Category: Phototropin]]
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[[Category: Moglich, A.]]
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[[Category: RCSB PDB Molecule of the Month]]
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[[Category: ACY]]
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[[Category: Moffat K]]
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[[Category: FMN]]
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[[Category: Moglich A]]
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[[Category: NA]]
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[[Category: flavoprotein]]
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[[Category: light-oxygen-voltage]]
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[[Category: lov]]
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[[Category: pas]]
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[[Category: per-arnt-sim]]
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[[Category: signaling protein]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 13:37:35 2007''
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Current revision

Structural Basis for Light-dependent Signaling in the Dimeric LOV Photosensor YtvA (Dark Structure)

PDB ID 2pr5

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