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2q16

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[[Image:2q16.png|left|200px]]
 
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{{STRUCTURE_2q16| PDB=2q16 | SCENE= }}
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==Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with ITP==
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<StructureSection load='2q16' size='340' side='right'caption='[[2q16]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
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===Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with ITP===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[2q16]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q16 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q16 FirstGlance]. <br>
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{{ABSTRACT_PUBMED_17976651}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ITT:INOSINE+5-TRIPHOSPHATE'>ITT</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q16 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q16 OCA], [https://pdbe.org/2q16 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q16 RCSB], [https://www.ebi.ac.uk/pdbsum/2q16 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q16 ProSAT]</span></td></tr>
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[[2q16]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q16 OCA].
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</table>
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== Function ==
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==Reference==
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[https://www.uniprot.org/uniprot/IXTPA_ECOLI IXTPA_ECOLI] Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP (PubMed:12297000, PubMed:17976651). Can also efficiently hydrolyze 2'-deoxy-N-6-hydroxylaminopurine triphosphate (dHAPTP) (PubMed:17090528). Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions (PubMed:12297000, PubMed:12730170, PubMed:17090528). To a much lesser extent, is also able to hydrolyze GTP, dGTP and dUTP, but shows very low activity toward the canonical nucleotides dATP, dCTP and dTTP and toward 8-oxo-dGTP, purine deoxyribose triphosphate, 2-aminopurine deoxyribose triphosphate and 2,6-diaminopurine deoxyribose triphosphate (PubMed:12297000, PubMed:17090528).[HAMAP-Rule:MF_01405]<ref>PMID:12297000</ref> <ref>PMID:12730170</ref> <ref>PMID:17090528</ref> <ref>PMID:17976651</ref>
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<ref group="xtra">PMID:017976651</ref><references group="xtra"/>
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== Evolutionary Conservation ==
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[[Category: Escherichia coli]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Nucleoside-triphosphate diphosphatase]]
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Check<jmol>
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[[Category: Lam, R.]]
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<jmolCheckbox>
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[[Category: Proudfoot, M.]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q1/2q16_consurf.spt"</scriptWhenChecked>
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[[Category: Savchenko, A.]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: Singer, A U.]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: Skarina, T.]]
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</jmolCheckbox>
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[[Category: Yakunin, A F.]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q16 ConSurf].
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[[Category: Hydrolase]]
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<div style="clear:both"></div>
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[[Category: Itp pyrophosphatase x-ray structure enzyme mechanism substrate]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Escherichia coli K-12]]
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[[Category: Large Structures]]
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[[Category: Lam R]]
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[[Category: Proudfoot M]]
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[[Category: Savchenko A]]
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[[Category: Singer AU]]
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[[Category: Skarina T]]
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[[Category: Yakunin AF]]

Current revision

Structure of the E. coli inosine triphosphate pyrophosphatase RgdB in complex with ITP

PDB ID 2q16

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