This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2q6t

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (09:14, 21 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2q6t.gif|left|200px]]
 
-
{{Structure
+
==Crystal structure of the Thermus aquaticus DnaB monomer==
-
|PDB= 2q6t |SIZE=350|CAPTION= <scene name='initialview01'>2q6t</scene>, resolution 2.90&Aring;
+
<StructureSection load='2q6t' size='340' side='right'caption='[[2q6t]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
-
|SITE=
+
== Structural highlights ==
-
|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
+
<table><tr><td colspan='2'>[[2q6t]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6T OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q6T FirstGlance]. <br>
-
|ACTIVITY=
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
-
|GENE= dnab ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=271 Thermus aquaticus])
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-
|DOMAIN=
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6t OCA], [https://pdbe.org/2q6t PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q6t RCSB], [https://www.ebi.ac.uk/pdbsum/2q6t PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q6t ProSAT]</span></td></tr>
-
|RELATEDENTRY=
+
</table>
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2q6t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q6t OCA], [http://www.ebi.ac.uk/pdbsum/2q6t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2q6t RCSB]</span>
+
== Function ==
-
}}
+
[https://www.uniprot.org/uniprot/Q9Z693_THEAQ Q9Z693_THEAQ]
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q6/2q6t_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q6t ConSurf].
 +
<div style="clear:both"></div>
-
'''Crystal structure of the Thermus aquaticus DnaB monomer'''
+
==See Also==
-
 
+
*[[Helicase 3D structures|Helicase 3D structures]]
-
 
+
__TOC__
-
==Overview==
+
</StructureSection>
-
The ring-shaped hexameric DnaB helicase unwinds duplex DNA at the replication fork of eubacteria. We have solved the crystal structure of the full-length Thermus aquaticus DnaB monomer, or possibly dimer, at 2.9 A resolution. DnaB is a highly flexible two domain protein. The C-terminal domain exhibits a RecA-like core fold and contains all the conserved sequence motifs that are characteristic of the DnaB helicase family. The N-terminal domain contains an additional helical hairpin that makes it larger than previously appreciated. Several DnaB mutations that modulate its interaction with primase are found in this hairpin. The similarity in the fold of the DnaB N-terminal domain with that of the C-terminal helicase-binding domain (HBD) of the DnaG primase also includes this hairpin. Comparison of hexameric homology models of DnaB with the structure of the papillomavirus E1 helicase suggests the two helicases may function through different mechanisms despite their sharing a common ancestor.
+
[[Category: Large Structures]]
-
 
+
-
==About this Structure==
+
-
2Q6T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q6T OCA].
+
-
 
+
-
==Reference==
+
-
The crystal structure of the Thermus aquaticus DnaB helicase monomer., Bailey S, Eliason WK, Steitz TA, Nucleic Acids Res. 2007;35(14):4728-36. Epub 2007 Jul 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17606462 17606462]
+
-
[[Category: Single protein]]
+
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
-
[[Category: Bailey, S.]]
+
[[Category: Bailey S]]
-
[[Category: Eliason, W K.]]
+
[[Category: Eliason WK]]
-
[[Category: Steitz, T A.]]
+
[[Category: Steitz TA]]
-
[[Category: dnab]]
+
-
[[Category: helicase]]
+
-
[[Category: replication]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:45:33 2008''
+

Current revision

Crystal structure of the Thermus aquaticus DnaB monomer

PDB ID 2q6t

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2q6t"
Personal tools