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2qh7

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[[Image:2qh7.jpg|left|200px]]<br /><applet load="2qh7" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2qh7, resolution 1.500&Aring;" />
 
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'''MitoNEET is a uniquely folded 2Fe-2S outer mitochondrial membrane protein stabilized by pioglitazone'''<br />
 
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==Overview==
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==MitoNEET is a uniquely folded 2Fe-2S outer mitochondrial membrane protein stabilized by pioglitazone==
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Iron-sulfur (Fe-S) proteins are key players in vital processes involving energy homeostasis and metabolism from the simplest to most complex organisms. We report a 1.5 A x-ray crystal structure of the first identified outer mitochondrial membrane Fe-S protein, mitoNEET. Two protomers intertwine to form a unique dimeric structure that constitutes a new fold to not only the approximately 650 reported Fe-S protein structures but also to all known proteins. We name this motif the NEET fold. The protomers form a two-domain structure: a beta-cap domain and a cluster-binding domain that coordinates two acid-labile 2Fe-2S clusters. Binding of pioglitazone, an insulin-sensitizing thiazolidinedione used in the treatment of type 2 diabetes, stabilizes the protein against 2Fe-2S cluster release. The biophysical properties of mitoNEET suggest that it may participate in a redox-sensitive signaling and/or in Fe-S cluster transfer.
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<StructureSection load='2qh7' size='340' side='right'caption='[[2qh7]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2qh7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QH7 FirstGlance]. <br>
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2QH7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=FES:'>FES</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QH7 OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qh7 OCA], [https://pdbe.org/2qh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2qh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qh7 ProSAT]</span></td></tr>
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MitoNEET is a uniquely folded 2Fe 2S outer mitochondrial membrane protein stabilized by pioglitazone., Paddock ML, Wiley SE, Axelrod HL, Cohen AE, Roy M, Abresch EC, Capraro D, Murphy AN, Nechushtai R, Dixon JE, Jennings PA, Proc Natl Acad Sci U S A. 2007 Sep 4;104(36):14342-7. Epub 2007 Aug 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17766440 17766440]
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CISD1_HUMAN CISD1_HUMAN] Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (By similarity). May be involved in Fe-S cluster shuttling and/or in redox reactions.<ref>PMID:17584744</ref> <ref>PMID:17766440</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qh/2qh7_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qh7 ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Axelrod, H L.]]
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[[Category: Abresch EC]]
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[[Category: Capraro, D.]]
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[[Category: Axelrod HL]]
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[[Category: Cohen, A E.]]
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[[Category: Capraro D]]
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[[Category: Dixon, J E.]]
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[[Category: Cohen AE]]
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[[Category: E C., Abresch..]]
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[[Category: Dixon JE]]
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[[Category: Jennings, P A.]]
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[[Category: Jennings PA]]
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[[Category: Murphy, A N.]]
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[[Category: Murphy AN]]
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[[Category: Nechushtai, R.]]
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[[Category: Nechushtai R]]
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[[Category: Paddock, M L.]]
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[[Category: Paddock ML]]
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[[Category: Roy, M.]]
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[[Category: Roy M]]
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[[Category: Wiley, S E.]]
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[[Category: Wiley SE]]
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[[Category: FES]]
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[[Category: 2fe-2s protein]]
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[[Category: metal binding protein]]
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[[Category: mitoneet]]
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[[Category: outer mitochrodrial membrane protein]]
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[[Category: pioglitazone binding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:39:24 2008''
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Current revision

MitoNEET is a uniquely folded 2Fe-2S outer mitochondrial membrane protein stabilized by pioglitazone

PDB ID 2qh7

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