This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2qvj

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /><applet load="2qvj" size="350" color="white" frame="true" align="right" spinBox="true" caption="2qvj, resolution 2.800&Aring;" /> '''Crystal structure o...)
Current revision (09:18, 21 February 2024) (edit) (undo)
 
(14 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:2qvj.jpg|left|200px]]<br /><applet load="2qvj" size="350" color="white" frame="true" align="right" spinBox="true"
 
-
caption="2qvj, resolution 2.800&Aring;" />
 
-
'''Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant'''<br />
 
-
==Overview==
+
==Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant==
-
The crystal structure of the vesicular stomatitis virus nucleoprotein (N), in complex with RNA reveals extensive and specific intermolecular, interactions among the N molecules in the 10-member oligomer. What roles, these interactions play in encapsidating RNA was studied by mutagenesis of, the N protein. Three N mutants intended for disruption of the, intermolecular interactions were designed and coexpressed with the, phosphoprotein (P) in an Escherichia coli system previously described (T., J. Green et al., J. Virol. 74:9515-9524, 2000). Mutants N (Delta1-22), N, (Delta347-352), and N (320-324, (Ala)(5)) lost RNA encapsidation and, oligomerization but still bound with P. Another mutant, N (Ser290--&gt;Trp), was able to form a stable ring-like N oligomer and bind with the P protein, but was no longer able to encapsidate RNA. The crystal structure of N, (Ser290--&gt;Trp) at 2.8 A resolution showed that this mutant can maintain, all the same intermolecular interactions as the wild-type N except for a, slight unwinding of the N-terminal lobe. These results suggest that the, intermolecular contacts among the N molecules are required for, encapsidation of the viral RNA.
+
<StructureSection load='2qvj' size='340' side='right'caption='[[2qvj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[2qvj]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Vesicular_stomatitis_Indiana_virus Vesicular stomatitis Indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QVJ FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qvj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qvj OCA], [https://pdbe.org/2qvj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qvj RCSB], [https://www.ebi.ac.uk/pdbsum/2qvj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qvj ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/A6H4P1_9RHAB A6H4P1_9RHAB]
-
==About this Structure==
+
==See Also==
-
2QVJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vesicular_stomatitis_indiana_virus Vesicular stomatitis indiana virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QVJ OCA].
+
*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Role of intermolecular interactions of vesicular stomatitis virus nucleoprotein in RNA encapsidation., Zhang X, Green TJ, Tsao J, Qiu S, Luo M, J Virol. 2008 Jan;82(2):674-82. Epub 2007 Nov 14. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18003727 18003727]
+
[[Category: Large Structures]]
-
[[Category: Single protein]]
+
[[Category: Vesicular stomatitis Indiana virus]]
-
[[Category: Vesicular stomatitis indiana virus]]
+
[[Category: Green TJ]]
-
[[Category: Green, T.J.]]
+
[[Category: Luo M]]
-
[[Category: Luo, M.]]
+
[[Category: Qiu S]]
-
[[Category: Qiu, S.]]
+
[[Category: Tsao J]]
-
[[Category: Tsao, J.]]
+
[[Category: Zhang X]]
-
[[Category: Zhang, X.]]
+
-
[[Category: cytoplasm]]
+
-
[[Category: nucleocapsid]]
+
-
[[Category: ribonucleoprotein]]
+
-
[[Category: rna binding protein]]
+
-
[[Category: rna-binding]]
+
-
[[Category: viral nucleoprotein]]
+
-
[[Category: virion]]
+
-
 
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jan 23 11:15:34 2008''
+

Current revision

Crystal structure of a vesicular stomatitis virus nucleocapsid protein Ser290Trp mutant

PDB ID 2qvj

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2qvj"
Personal tools