2qzv

<StructureSection load='2qzv' size='340' side='right' caption='[[2qzv]], [[Resolution|resolution]] 9.00&Aring;' scene=''>

<table><tr><td colspan='2'>[[2qzv]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Buffalo_rat Buffalo rat]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QZV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2QZV FirstGlance]. <br>

</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1y7x|1y7x]]</td></tr>

<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mvp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Buffalo rat])</td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qzv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qzv OCA], [http://pdbe.org/2qzv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2qzv RCSB], [http://www.ebi.ac.uk/pdbsum/2qzv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2qzv ProSAT]</span></td></tr>

[[http://www.uniprot.org/uniprot/MVP_RAT MVP_RAT]] Required for normal vault structure. Vaults are multi-subunit structures that may act as scaffolds for proteins involved in signal transduction. Vaults may also play a role in nucleo-cytoplasmic transport. Down-regulates INFG-mediated STAT1 signaling and subsequent activation of JAK. Down-regulates SRC activity and signaling through MAP kinases (By similarity).

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== Publication Abstract from PubMed ==

Vaults are the largest known cytoplasmic ribonucleoprotein structures and may function in innate immunity. The vault shell self-assembles from 96 copies of major vault protein and encapsulates two other proteins and a small RNA. We crystallized rat liver vaults and several recombinant vaults, all among the largest non-icosahedral particles to have been crystallized. The best crystals thus far were formed from empty vaults built from a cysteine-tag construct of major vault protein (termed cpMVP vaults), diffracting to about 9-A resolution. The asymmetric unit contains a half vault of molecular mass 4.65 MDa. X-ray phasing was initiated by molecular replacement, using density from cryo-electron microscopy (cryo-EM). Phases were improved by density modification, including concentric 24- and 48-fold rotational symmetry averaging. From this, the continuous cryo-EM electron density separated into domain-like blocks. A draft atomic model of cpMVP was fit to this improved density from 15 domain models. Three domains were adapted from a nuclear magnetic resonance substructure. Nine domain models originated in ab initio tertiary structure prediction. Three C-terminal domains were built by fitting poly-alanine to the electron density. Locations of loops in this model provide sites to test vault functions and to exploit vaults as nanocapsules.

Draft crystal structure of the vault shell at 9-A resolution.,Anderson DH, Kickhoefer VA, Sievers SA, Rome LH, Eisenberg D PLoS Biol. 2007 Nov;5(11):e318. PMID:18044992<ref>PMID:18044992</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 2qzv" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Buffalo rat]]

[[Category: Anderson, D H]]

[[Category: Eisenberg, D]]

[[Category: Kickhoefer, V A]]

[[Category: Rome, L H]]

[[Category: Sievers, S A]]

[[Category: Barrel self-assembled from 96 stave]]

[[Category: Structural protein]]