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2r5o

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[[Image:2r5o.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the C-terminal domain of wzt==
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|PDB= 2r5o |SIZE=350|CAPTION= <scene name='initialview01'>2r5o</scene>, resolution 1.300&Aring;
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<StructureSection load='2r5o' size='340' side='right'caption='[[2r5o]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene> and <scene name='pdbligand=PG4:TETRAETHYLENE GLYCOL'>PG4</scene>
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<table><tr><td colspan='2'>[[2r5o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R5O FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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|GENE= ORF431 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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}}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r5o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5o OCA], [https://pdbe.org/2r5o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r5o RCSB], [https://www.ebi.ac.uk/pdbsum/2r5o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r5o ProSAT]</span></td></tr>
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</table>
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'''Crystal structure of the C-terminal domain of wzt'''
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== Function ==
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[https://www.uniprot.org/uniprot/Q47591_ECOLX Q47591_ECOLX]
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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ATP-binding-cassette (ABC) transporters are responsible for the export of a wide variety of cell-surface glycoconjugates in both Gram-positive and Gram-negative bacteria. These include the O-antigenic polysaccharide (O-PS) portion of lipopolysaccharide, a crucial virulence determinant in Gram-negative pathogens. O-PSs are synthesized by one of two fundamentally different pathways. Escherichia coli O serotypes O8 and O9a provide the prototype systems for studying O-PS export via ABC transporters. The transporter is composed of the transmembrane component Wzm and the nucleotide-binding component Wzt. Although the N-terminal domain of Wzt is a conventional ABC protein, the C-terminal domain of Wzt (C-Wzt) is a unique structural element that determines the specificity of the transporter for either the O8 or O9a O-PS. We show here that the two domains of Wzt can function when expressed as separate polypeptides; both are essential for export. In vitro, C-Wzt binds its cognate O-PS by recognizing a residue located at the nonreducing end of the polymer. The crystal structure of C-Wzt(O9a) is reported here and reveals a beta sandwich with an immunoglobulin-like topology that contains the O-PS-binding pocket. Substrate interactions with nucleotide-binding domains have been demonstrated in an ABC exporter previously. However, to our knowledge substrate binding by a discrete, cytoplasmic accessory domain in an extended nucleotide-binding domain polypeptide has not previously been demonstrated. Elucidation of the substrate-recognition system involved in O-PS export provides insight into the mechanism that coordinates polymer biosynthesis, termination, and export.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/2r5o_consurf.spt"</scriptWhenChecked>
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2R5O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5O OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Substrate binding by a bacterial ABC transporter involved in polysaccharide export., Cuthbertson L, Kimber MS, Whitfield C, Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19529-34. Epub 2007 Nov 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18032609 18032609]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r5o ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Cuthbertson, L.]]
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[[Category: Cuthbertson L]]
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[[Category: Kimber, M S.]]
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[[Category: Kimber MS]]
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[[Category: Whitfield, C.]]
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[[Category: Whitfield C]]
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[[Category: CL]]
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[[Category: NA]]
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[[Category: PG4]]
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[[Category: abc transporter]]
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[[Category: atp-binding]]
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[[Category: carbohydrate binding]]
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[[Category: domain swapping]]
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[[Category: immunoglobulin fold]]
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[[Category: nucleotide-binding]]
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[[Category: o antigen export]]
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[[Category: transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:32:47 2008''
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Current revision

Crystal structure of the C-terminal domain of wzt

PDB ID 2r5o

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