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2r5u

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(New page: 200px <!-- The line below this paragraph, containing "STRUCTURE_2r5u", creates the "Structure Box" on the page. You may change the PDB parameter (which sets the PD...)
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[[Image:2r5u.jpg|left|200px]]
 
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==Crystal structure of the N-terminal domain of DnaB helicase from Mycobacterium tuberculosis==
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The line below this paragraph, containing "STRUCTURE_2r5u", creates the "Structure Box" on the page.
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<StructureSection load='2r5u' size='340' side='right'caption='[[2r5u]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[2r5u]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R5U FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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{{STRUCTURE_2r5u| PDB=2r5u | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5u OCA], [https://pdbe.org/2r5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r5u RCSB], [https://www.ebi.ac.uk/pdbsum/2r5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r5u ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNAB_MYCTU DNAB_MYCTU] Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity (By similarity). The intein is an endonuclease (Potential).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/2r5u_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r5u ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the N-terminal domain of DnaB helicase from Mycobacterium tuberculosis'''
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==See Also==
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*[[Helicase 3D structures|Helicase 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Hexameric DnaB helicase unwinds the DNA double helix during replication of genetic material in bacteria. DnaB is an essential bacterial protein; therefore, it is an important potential target for antibacterial drug discovery. We report a crystal structure of the N-terminal region of DnaB from the pathogen Mycobacterium tuberculosis (MtDnaBn), determined at 2.0 A resolution. This structure provides atomic resolution details of formation of the hexameric ring of DnaB by two distinct interfaces. An extensive hydrophobic interface stabilizes a dimer of MtDnaBn by forming a four-helix bundle. The other, less extensive, interface is formed between the dimers, connecting three of them into a hexameric ring. On the basis of crystal packing interactions between MtDnaBn rings, we suggest a model of a helicase-primase complex that explains previously observed effects of DnaB mutations on DNA priming.
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[[Category: Large Structures]]
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[[Category: Mycobacterium tuberculosis H37Rv]]
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==About this Structure==
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[[Category: Biswas T]]
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2R5U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5U OCA].
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[[Category: Tsodikov OV]]
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==Reference==
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Hexameric ring structure of the N-terminal domain of Mycobacterium tuberculosis DnaB helicase., Biswas T, Tsodikov OV, FEBS J. 2008 Jun;275(12):3064-71. Epub 2008 May 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18479467 18479467]
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[[Category: Mycobacterium tuberculosis]]
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[[Category: Single protein]]
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[[Category: Biswas, T.]]
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[[Category: Tsodikov, O V.]]
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[[Category: Atp-binding]]
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[[Category: Autocatalytic cleavage]]
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[[Category: Dna replication]]
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[[Category: Dna-binding]]
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[[Category: Dnab]]
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[[Category: Endonuclease]]
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[[Category: Helicase]]
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[[Category: Hydrolase]]
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[[Category: Intron homing]]
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[[Category: Nuclease]]
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[[Category: Nucleotide-binding]]
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[[Category: Primase]]
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[[Category: Primosome]]
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[[Category: Protein splicing]]
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[[Category: Replication]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:14:29 2008''
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Current revision

Crystal structure of the N-terminal domain of DnaB helicase from Mycobacterium tuberculosis

PDB ID 2r5u

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