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2rcj

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[[Image:2rcj.jpg|left|200px]]<br /><applet load="2rcj" size="350" color="white" frame="true" align="right" spinBox="true"
 
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caption="2rcj" />
 
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'''Solution structure of human Immunoglobulin M'''<br />
 
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==Overview==
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==Solution structure of human Immunoglobulin M==
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IgA is unique in being able to form a diverse range of polymeric structures. Increases in the levels of dimeric IgA1 (dIgA1) in serum have been implicated in diseases such as IgA nephropathy. We have determined the solution structure for dIgA1 by synchrotron x-ray and neutron scattering and analytical ultracentrifugation. The Guinier radius of gyration (RG) of 7.60-8.65 nm indicated that the two monomers within dIgA1 are arranged in an extended conformation. The distance distribution curve P(r) gave an overall length (L) of 22-26 nm. These results were confirmed by the sedimentation coefficient and frictional ratio of dIgA1. Constrained scattering modeling starting from the IgA1 monomer solution structure revealed a near-planar dimer structure for dIgA1. The two Fc regions form a slightly bent arrangement in which they form end-to-end contacts, and the J chain was located at this interface. This structure was refined by optimizing the position of the four Fab regions. From this, the best-fit solution structures show that the four Fab Ag-binding sites are independent of one another, and the two Fc regions are accessible to receptor binding. This arrangement allows dIgA1 to initiate specific immune responses by binding to FcalphaRI receptors, while still retaining Ag-binding ability, and to be selectively transported to mucosal surfaces by binding to the polymeric Ig receptor to form secretory IgA. A mechanism for the involvement of dIgA1 oligomers in the pathology of IgA nephropathy is discussed in the light of this near-planar structure.
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<StructureSection load='2rcj' size='340' side='right'caption='[[2rcj]]' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[2rcj]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RCJ FirstGlance]. <br>
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2RCJ is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCJ OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray solution scattering</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rcj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcj OCA], [https://pdbe.org/2rcj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rcj RCSB], [https://www.ebi.ac.uk/pdbsum/2rcj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rcj ProSAT]</span></td></tr>
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==Reference==
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</table>
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Implications of the near-planar solution structure of human myeloma dimeric IgA1 for mucosal immunity and IgA nephropathy., Bonner A, Furtado PB, Almogren A, Kerr MA, Perkins SJ, J Immunol. 2008 Jan 15;180(2):1008-18. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=18178841 18178841]
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== Function ==
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[https://www.uniprot.org/uniprot/Q6PYX1_HUMAN Q6PYX1_HUMAN]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/2rcj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rcj ConSurf].
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Feinstein, A.]]
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[[Category: Feinstein A]]
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[[Category: Nealis, A S.]]
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[[Category: Nealis AS]]
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[[Category: Perkins, S J.]]
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[[Category: Perkins SJ]]
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[[Category: Sutton, B J.]]
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[[Category: Sutton BJ]]
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[[Category: immune system]]
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[[Category: immunoglobulin m; polymeric antibodies; immunology; x-ray solution scattering; constrained modelling]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 18:46:18 2008''
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Current revision

Solution structure of human Immunoglobulin M

PDB ID 2rcj

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