3b9y

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[[Image:3b9y.jpg|left|200px]]
 
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{{Structure
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==Crystal structure of the Nitrosomonas europaea Rh protein==
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|PDB= 3b9y |SIZE=350|CAPTION= <scene name='initialview01'>3b9y</scene>, resolution 1.850&Aring;
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<StructureSection load='3b9y' size='340' side='right'caption='[[3b9y]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene>
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<table><tr><td colspan='2'>[[3b9y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Nitrosomonas_europaea_ATCC_19718 Nitrosomonas europaea ATCC 19718]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B9Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B9Y FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
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|GENE= Rh50, NE0448 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228410 Nitrosomonas europaea ATCC 19718])
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
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|DOMAIN=
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b9y OCA], [https://pdbe.org/3b9y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b9y RCSB], [https://www.ebi.ac.uk/pdbsum/3b9y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b9y ProSAT]</span></td></tr>
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|RELATEDENTRY=[[3b9z|3B9Z]]
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</table>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3b9y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b9y OCA], [http://www.ebi.ac.uk/pdbsum/3b9y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3b9y RCSB]</span>
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== Function ==
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}}
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[https://www.uniprot.org/uniprot/Q82X47_NITEU Q82X47_NITEU]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b9/3b9y_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b9y ConSurf].
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<div style="clear:both"></div>
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'''Crystal structure of the Nitrosomonas europaea Rh protein'''
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==See Also==
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*[[Ion channels 3D structures|Ion channels 3D structures]]
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__TOC__
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==Overview==
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</StructureSection>
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Amt/MEP/Rh proteins are a family of integral membrane proteins implicated in the transport of NH3, CH(2)NH2, and CO2. Whereas Amt/MEP proteins are agreed to transport ammonia (NH3/NH4+), the primary substrate for Rh proteins has been controversial. Initial studies suggested that Rh proteins also transport ammonia, but more recent evidence suggests that they transport CO2. Here we report the first structure of an Rh family member, the Rh protein from the chemolithoautotrophic ammonia-oxidizing bacterium Nitrosomonas europaea. This Rh protein exhibits a number of similarities to its Amt cousins, including a trimeric oligomeric state, a central pore with an unusual twin-His site in the middle, and a Phe residue that blocks the channel for small-molecule transport. However, there are some significant differences, the most notable being the presence of an additional cytoplasmic C-terminal alpha-helix, an increased number of internal proline residues along the transmembrane helices, and a specific set of residues that appear to link the C-terminal helix to Phe blockage. This latter linkage suggests a mechanism in which binding of a partner protein to the C terminus could regulate channel opening. Another difference is the absence of the extracellular pi-cation binding site conserved in Amt/Mep structures. Instead, CO2 pressurization experiments identify a CO2 binding site near the intracellular exit of the channel whose residues are highly conserved in all Rh proteins, except those belonging to the Rh30 subfamily. The implications of these findings on the functional role of the human Rh antigens are discussed.
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[[Category: Large Structures]]
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[[Category: Nitrosomonas europaea ATCC 19718]]
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==About this Structure==
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[[Category: Chan MK]]
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3B9Y is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Nitrosomonas_europaea_atcc_19718 Nitrosomonas europaea atcc 19718]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B9Y OCA].
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[[Category: Jayachandran S]]
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[[Category: Li X]]
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==Reference==
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[[Category: Nguyen H-HT]]
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Structure of the Nitrosomonas europaea Rh protein., Li X, Jayachandran S, Nguyen HH, Chan MK, Proc Natl Acad Sci U S A. 2007 Dec 4;104(49):19279-84. Epub 2007 Nov 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18040042 18040042]
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[[Category: Nitrosomonas europaea atcc 19718]]
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[[Category: Single protein]]
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[[Category: Chan, M K.]]
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[[Category: Jayachandran, S.]]
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[[Category: Li, X.]]
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[[Category: Nguyen, H-H T.]]
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[[Category: carbon dioxide]]
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[[Category: channel]]
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[[Category: co2]]
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[[Category: rh protein]]
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[[Category: transport protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:24:32 2008''
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Current revision

Crystal structure of the Nitrosomonas europaea Rh protein

PDB ID 3b9y

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