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3brf

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[[Image:3brf.jpg|left|200px]]
 
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==CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221==
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The line below this paragraph, containing "STRUCTURE_3brf", creates the "Structure Box" on the page.
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<StructureSection load='3brf' size='340' side='right'caption='[[3brf]], [[Resolution|resolution]] 2.47&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3brf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BRF FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.47&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SOR:D-SORBITOL'>SOR</scene></td></tr>
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{{STRUCTURE_3brf| PDB=3brf | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3brf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3brf OCA], [https://pdbe.org/3brf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3brf RCSB], [https://www.ebi.ac.uk/pdbsum/3brf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3brf ProSAT]</span></td></tr>
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</table>
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'''CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221'''
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== Function ==
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[https://www.uniprot.org/uniprot/LIN12_CAEEL LIN12_CAEEL] Involved in several cell fate decisions that require cell-cell interactions. It is possible that lin-12 encodes a membrane-bound receptor for a signal that enables expression of the ventral uterine precursor cell fate. Activity in cell fate decisions and tumorigenesis is negatively regulated by sel-10.<ref>PMID:3419531</ref> <ref>PMID:3000611</ref>
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/br/3brf_consurf.spt"</scriptWhenChecked>
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3BRF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BRF OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18381292 18381292]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3brf ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Caenorhabditis elegans]]
[[Category: Caenorhabditis elegans]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Kovall, R A.]]
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[[Category: Kovall RA]]
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[[Category: Wilson, J J.]]
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[[Category: Wilson JJ]]
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[[Category: Ank repeat]]
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[[Category: Developmental protein]]
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[[Category: Differentiation]]
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[[Category: Dna binding protein/dna complex]]
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[[Category: Dna-binding]]
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[[Category: Egf-like domain]]
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[[Category: Glycoprotein]]
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[[Category: Membrane]]
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[[Category: Notch]]
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[[Category: Protein-dna complex]]
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[[Category: Signaling]]
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[[Category: Transcription]]
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[[Category: Transmembrane]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Apr 24 09:33:19 2008''
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Current revision

CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221

PDB ID 3brf

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