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3bxj

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[[Image:3bxj.jpg|left|200px]]
 
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{{Structure
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==Crystal Structure of the C2-GAP Fragment of synGAP==
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|PDB= 3bxj |SIZE=350|CAPTION= <scene name='initialview01'>3bxj</scene>, resolution 3.0&Aring;
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<StructureSection load='3bxj' size='340' side='right'caption='[[3bxj]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
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|SITE=
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== Structural highlights ==
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|LIGAND=
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<table><tr><td colspan='2'>[[3bxj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BXJ FirstGlance]. <br>
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|ACTIVITY=
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
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|GENE= Syngap1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bxj OCA], [https://pdbe.org/3bxj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bxj RCSB], [https://www.ebi.ac.uk/pdbsum/3bxj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bxj ProSAT]</span></td></tr>
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|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam00616 RasGAP], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=smart00323 RasGAP], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=smart00239 C2]</span>
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</table>
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|RELATEDENTRY=
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== Function ==
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3bxj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bxj OCA], [http://www.ebi.ac.uk/pdbsum/3bxj PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=3bxj RCSB]</span>
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[https://www.uniprot.org/uniprot/SYGP1_RAT SYGP1_RAT] Major constituent of the PSD essential for postsynaptic signaling. Inhibitory regulator of the Ras-cAMP pathway. Member of the NMDAR signaling complex in excitatory synapses, it may play a role in NMDAR-dependent control of AMPAR potentiation, AMPAR membrane trafficking and synaptic plasticity. Regulates AMPAR-mediated miniature excitatory postsynaptic currents. May be involved in certain forms of brain injury, leading to long-term learning and memory deficits.<ref>PMID:15312654</ref> <ref>PMID:15500970</ref> <ref>PMID:16537406</ref>
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}}
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bx/3bxj_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bxj ConSurf].
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<div style="clear:both"></div>
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'''Crystal Structure of the C2-GAP Fragment of synGAP'''
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==See Also==
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*[[Ras GTPase activating protein|Ras GTPase activating protein]]
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== References ==
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==Overview==
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<references/>
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The brain-specific synaptic guanosine triphosphatase (GTPase)-activating protein (SynGAP) is important in synaptic plasticity. It shows dual specificity for the small guanine nucleotide-binding proteins Rap and Ras. Here, we show that RapGAP activity of SynGAP requires its C2 domain. In contrast to the isolated GAP domain, which does not show any detectable RapGAP activity, a fragment comprising the C2 and GAP domains (C2-GAP) stimulates the intrinsic GTPase reaction of Rap by approximately 1 x 10(4). The C2-GAP crystal structure, complemented by modelling and biochemical analyses, favours a concerted movement of the C2 domain towards the switch II region of Rap to assist in GTPase stimulation. Our data support a catalytic mechanism similar to that of canonical RasGAPs and distinct from the canonical RapGAPs. SynGAP presents the first example, to our knowledge, of a GAP that uses a second domain for catalytic activity, thus pointing to a new function of C2 domains.
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__TOC__
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</StructureSection>
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==About this Structure==
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[[Category: Large Structures]]
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3BXJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BXJ OCA].
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==Reference==
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The C2 domain of SynGAP is essential for stimulation of the Rap GTPase reaction., Pena V, Hothorn M, Eberth A, Kaschau N, Parret A, Gremer L, Bonneau F, Ahmadian MR, Scheffzek K, EMBO Rep. 2008 Mar 7;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18323856 18323856]
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[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
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[[Category: Single protein]]
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[[Category: Ahmadian MR]]
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[[Category: Ahmadian, M R.]]
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[[Category: Bonneau F]]
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[[Category: Bonneau, F.]]
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[[Category: Eberth A]]
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[[Category: Eberth, A.]]
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[[Category: Gremer L]]
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[[Category: Gremer, L.]]
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[[Category: Hothorn M]]
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[[Category: Hothorn, M.]]
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[[Category: Kaschau N]]
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[[Category: Kaschau, N.]]
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[[Category: Parret A]]
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[[Category: Parret, A.]]
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[[Category: Pena V]]
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[[Category: Pena, V.]]
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[[Category: Scheffzek K]]
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[[Category: Scheffzek, K.]]
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[[Category: alternative splicing]]
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[[Category: gtpase activating protein]]
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[[Category: gtpase activation]]
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[[Category: membrane]]
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[[Category: phosphoprotein]]
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[[Category: sh3-binding]]
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[[Category: signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:29:33 2008''
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Current revision

Crystal Structure of the C2-GAP Fragment of synGAP

PDB ID 3bxj

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