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3cnc

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[[Image:3cnc.jpg|left|200px]]
 
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==Crystal Structure of Ad16 fiber knob==
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The line below this paragraph, containing "STRUCTURE_3cnc", creates the "Structure Box" on the page.
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<StructureSection load='3cnc' size='340' side='right'caption='[[3cnc]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cnc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_adenovirus_16 Human adenovirus 16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CNC FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cnc OCA], [https://pdbe.org/3cnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cnc RCSB], [https://www.ebi.ac.uk/pdbsum/3cnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cnc ProSAT]</span></td></tr>
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{{STRUCTURE_3cnc| PDB=3cnc | SCENE= }}
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</table>
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== Function ==
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'''Crystal Structure of Ad16 fiber knob'''
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[https://www.uniprot.org/uniprot/Q67711_ADE16 Q67711_ADE16]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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A majority of species B adenoviruses uses CD46 as their primary receptor, however the precise mechanisms involved in the binding of different Ad types to CD46 have not been resolved. Although previous studies indicate close similarities between two members of species B2 adenoviruses in their usage of CD46, our current investigations revealed a surprisingly low CD46 binding affinity of the species B1 Ad16 fiber knob (KD of 437 nM). We determined the crystal structure of Ad16 fiber knob and constructed a model of this protein in complex with CD46. Comparison of this model to that of the CD46-Ad11 complex revealed structural differences in the FG and IJ loops that are part of the CD46 binding site. Analysis of a panel of recombinant fiber knobs with mutations targeting these regions in Ad16 and Ad11 uncovered a major contribution of the FG loop on CD46 binding. Two extra residues in the FG loop of the Ad16 fiber significantly reduce receptor interaction. Although avidity effects permit Ad16 use of CD46 on host cells, virus binding occurs with lower efficiency than with B2 Ad types. The longer FG loop of the Ad16 fiber knob is also shared by other species B1 Ad fibers and thus may contribute to the low CD46 binding efficiencies observed for these Ad types. Our findings provide a better understanding of how different Ad types associate with CD46 and could aid the selection of specific Ad fibers for more efficient adenoviral gene delivery vectors.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/3cnc_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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3CNC is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_16 Human adenovirus 16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNC OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cnc ConSurf].
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Structural Variations in Species B Adenovirus Fibers Impact CD46 Association., Pache L, Venkataraman S, Reddy VS, Nemerow GR, J Virol. 2008 Jun 4;. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18524830 18524830]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
[[Category: Human adenovirus 16]]
[[Category: Human adenovirus 16]]
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[[Category: Single protein]]
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[[Category: Large Structures]]
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[[Category: Nemerow, G R.]]
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[[Category: Nemerow GR]]
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[[Category: Pache, L.]]
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[[Category: Pache L]]
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[[Category: Reddy, V S.]]
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[[Category: Reddy VS]]
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[[Category: Venkataraman, S.]]
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[[Category: Venkataraman S]]
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[[Category: Adenovirus fiber knob]]
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[[Category: Viral protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 18 12:11:01 2008''
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Current revision

Crystal Structure of Ad16 fiber knob

PDB ID 3cnc

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