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3cnf

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[[Image:3cnf.jpg|left|200px]]
 
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==3.88 Angstrom structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy==
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The line below this paragraph, containing "STRUCTURE_3cnf", creates the "Structure Box" on the page.
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<SX load='3cnf' size='340' side='right' viewer='molstar' caption='[[3cnf]], [[Resolution|resolution]] 3.88&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3cnf]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CNF FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.88&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3cnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3cnf OCA], [https://pdbe.org/3cnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3cnf RCSB], [https://www.ebi.ac.uk/pdbsum/3cnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3cnf ProSAT]</span></td></tr>
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{{STRUCTURE_3cnf| PDB=3cnf | SCENE= }}
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q9E957_CPVBM Q9E957_CPVBM]
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cn/3cnf_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3cnf ConSurf].
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<div style="clear:both"></div>
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'''3.88 Angstrom structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy'''
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==See Also==
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*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
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__TOC__
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==Overview==
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</SX>
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Cytoplasmic polyhedrosis virus (CPV) is unique within the Reoviridae family in having a turreted single-layer capsid contained within polyhedrin inclusion bodies, yet being fully capable of cell entry and endogenous RNA transcription. Biochemical data have shown that the amino-terminal 79 residues of the CPV turret protein (TP) is sufficient to bring CPV or engineered proteins into the polyhedrin matrix for micro-encapsulation. Here we report the three-dimensional structure of CPV at 3.88 A resolution using single-particle cryo-electron microscopy. Our map clearly shows the turns and deep grooves of alpha-helices, the strand separation in beta-sheets, and densities for loops and many bulky side chains; thus permitting atomic model-building effort from cryo-electron microscopy maps. We observed a helix-to-beta-hairpin conformational change between the two conformational states of the capsid shell protein in the region directly interacting with genomic RNA. We have also discovered a messenger RNA release hole coupled with the mRNA capping machinery unique to CPV. Furthermore, we have identified the polyhedrin-binding domain, a structure that has potential in nanobiotechnology applications.
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==About this Structure==
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3CNF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Bombyx_mori_cypovirus_1 Bombyx mori cypovirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CNF OCA].
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==Reference==
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3.88 A structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy., Yu X, Jin L, Zhou ZH, Nature. 2008 May 15;453(7193):415-9. Epub 2008 Apr 30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18449192 18449192]
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[[Category: Bombyx mori cypovirus 1]]
[[Category: Bombyx mori cypovirus 1]]
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[[Category: Protein complex]]
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[[Category: Large Structures]]
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[[Category: Jin, L.]]
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[[Category: Jin L]]
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[[Category: Yu, X.]]
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[[Category: Yu X]]
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[[Category: Zhou, Z H.]]
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[[Category: Zhou ZH]]
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[[Category: Capsid protein]]
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[[Category: Cytoplasmic polyhedrosis virus]]
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[[Category: Guanylyltransferase domain]]
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[[Category: Icosahedral virus]]
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[[Category: Polyhedrin-binding domain]]
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[[Category: Turret protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu May 22 22:37:38 2008''
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Current revision

3.88 Angstrom structure of cytoplasmic polyhedrosis virus by cryo-electron microscopy

3cnf, resolution 3.88Å

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