3crm

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[[Image:3crm.jpg|left|200px]]
 
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==Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel==
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The line below this paragraph, containing "STRUCTURE_3crm", creates the "Structure Box" on the page.
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<StructureSection load='3crm' size='340' side='right'caption='[[3crm]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[3crm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CRM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3CRM FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3crm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3crm OCA], [https://pdbe.org/3crm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3crm RCSB], [https://www.ebi.ac.uk/pdbsum/3crm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3crm ProSAT]</span></td></tr>
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{{STRUCTURE_3crm| PDB=3crm | SCENE= }}
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</table>
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== Function ==
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'''Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel'''
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[https://www.uniprot.org/uniprot/MIAA_PSEAE MIAA_PSEAE] Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A).
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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==Overview==
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Check<jmol>
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Dimethylallyltransferase (DMATase) transfers a five-carbon isoprenoid moiety from dimethylallyl pyrophosphate (DMAPP) to the amino group of adenosine at position 37 of certain tRNAs. Reported here are the crystal structures of Pseudomonas aeruginosa DMATase alone and in complex with pyrophosphate at 1.9 A resolution. Surprisingly, the enzyme possesses a central channel spanning the entire width of the enzyme. Both the accepting substrate tRNA and the donating substrate DMAPP appear to enter the channel from opposite sides in an ordered sequence, with tRNA first and DMAPP second, and the RNA modification reaction occurs in the middle of the channel once the two substrates have met. The structure of DMATase is homologous to a class of small soluble kinases involved in biosynthesis of nucleotide precursors for nucleic acids, indicating its possibly evolutionary origin. Furthermore, specific recognition of the pyrophosphate by a conserved loop in DMATase, similar to the P-loop commonly seen in diverse nucleotide-binding proteins, demonstrates that DMATase is structurally and mechanistically distinct from farnesyltransferase, another family of prenyltransferases involved in protein modification.
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cr/3crm_consurf.spt"</scriptWhenChecked>
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==About this Structure==
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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3CRM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3CRM OCA].
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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==Reference==
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3crm ConSurf].
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Structure of tRNA dimethylallyltransferase: RNA modification through a channel., Xie W, Zhou C, Huang RH, J Mol Biol. 2007 Mar 30;367(3):872-81. Epub 2007 Jan 24. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17292915 17292915]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
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[[Category: Single protein]]
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[[Category: Huang RH]]
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[[Category: TRNA isopentenyltransferase]]
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[[Category: Xie W]]
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[[Category: Huang, R H.]]
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[[Category: Zhou C]]
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[[Category: Xie, W.]]
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[[Category: Zhou, C.]]
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[[Category: Atp-binding]]
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[[Category: Nucleotide-binding]]
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[[Category: Nucleotidyltransferase]]
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[[Category: Transferase]]
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[[Category: Trna processing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Apr 30 13:44:08 2008''
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Current revision

Structure of tRNA Dimethylallyltransferase: RNA Modification through a Channel

PDB ID 3crm

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