3hlc

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[[Image:3hlc.png|left|200px]]
 
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{{STRUCTURE_3hlc| PDB=3hlc | SCENE= }}
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==Simvastatin Synthase (LovD) from Aspergillus terreus, S5 mutant, unliganded==
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<StructureSection load='3hlc' size='340' side='right'caption='[[3hlc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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===Simvastatin Synthase (LovD) from Aspergillus terreus, S5 mutant, unliganded===
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== Structural highlights ==
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<table><tr><td colspan='2'>[[3hlc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3HLC FirstGlance]. <br>
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{{ABSTRACT_PUBMED_19875080}}
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
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==About this Structure==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3hlc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3hlc OCA], [https://pdbe.org/3hlc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3hlc RCSB], [https://www.ebi.ac.uk/pdbsum/3hlc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3hlc ProSAT]</span></td></tr>
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[[3hlc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_terreus Aspergillus terreus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3HLC OCA].
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/LOVD_ASPTE LOVD_ASPTE] Monacolin J acid methylbutanoyltransferase; part of the gene cluster that mediates the biosynthesis of lovastatin (also known as mevinolin, mevacor or monacolin K), a hypolipidemic inhibitor of (3S)-hydroxymethylglutaryl-coenzyme A (HMG-CoA) reductase (HMGR) (PubMed:10334994, PubMed:12929390, PubMed:21495633). The first step in the biosynthesis of lovastatin is the production of dihydromonacolin L acid by the lovastatin nonaketide synthase lovB and the trans-acting enoyl reductase lovC via condensation of one acetyl-CoA unit and 8 malonyl-CoA units (PubMed:10334994, PubMed:10381407, PubMed:19900898, PubMed:22733743). Dihydromonacolin L acid is released from lovB by the thioesterase lovG (PubMed:23653178). Next, dihydromonacolin L acid is oxidized by the dihydromonacolin L monooxygenase lovA twice to form monacolin J acid (PubMed:12929390, PubMed:21495633). The 2-methylbutyrate moiety of lovastatin is synthesized by the lovastatin diketide synthase lovF via condensation of one acetyl-CoA unit and one malonyl-CoA unit (PubMed:19530726, PubMed:21069965). Finally, the covalent attachment of this moiety to monacolin J acid is catalyzed by the transesterase lovD to yield lovastatin (PubMed:10334994, PubMed:17113998, PubMed:18988191, PubMed:19875080, PubMed:24727900). LovD has broad substrate specificity and can also convert monacolin J to simvastatin using alpha-dimethylbutanoyl-S-methyl-3-mercaptopropionate (DMB-S-MMP) as the thioester acyl donor, and can also catalyze the reverse reaction and function as hydrolase in vitro (PubMed:19875080). LovD has much higher activity with LovF-bound 2-methylbutanoate than with free diketide substrates (PubMed:21069965).<ref>PMID:10334994</ref> <ref>PMID:10381407</ref> <ref>PMID:12929390</ref> <ref>PMID:17113998</ref> <ref>PMID:18988191</ref> <ref>PMID:19530726</ref> <ref>PMID:19875080</ref> <ref>PMID:19900898</ref> <ref>PMID:21069965</ref> <ref>PMID:21495633</ref> <ref>PMID:22733743</ref> <ref>PMID:23653178</ref> <ref>PMID:24727900</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hl/3hlc_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3hlc ConSurf].
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<div style="clear:both"></div>
==See Also==
==See Also==
*[[Simvastatin Synthase|Simvastatin Synthase]]
*[[Simvastatin Synthase|Simvastatin Synthase]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:019875080</ref><references group="xtra"/>
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__TOC__
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</StructureSection>
[[Category: Aspergillus terreus]]
[[Category: Aspergillus terreus]]
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[[Category: Gao, X.]]
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[[Category: Large Structures]]
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[[Category: Laidman, J.]]
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[[Category: Gao X]]
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[[Category: Pashkov, I.]]
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[[Category: Laidman J]]
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[[Category: Sawaya, M R.]]
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[[Category: Pashkov I]]
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[[Category: Tang, Y.]]
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[[Category: Sawaya MR]]
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[[Category: Yeates, T O.]]
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[[Category: Tang Y]]
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[[Category: Alpha/beta hydrolase fold]]
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[[Category: Yeates TO]]
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[[Category: Transferase]]
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Current revision

Simvastatin Synthase (LovD) from Aspergillus terreus, S5 mutant, unliganded

PDB ID 3hlc

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OCA

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