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3ihg

From Proteopedia

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{{STRUCTURE_3ihg| PDB=3ihg | SCENE= }}
 
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===Crystal structure of a ternary complex of aklavinone-11 hydroxylase with FAD and aklavinone===
 
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{{ABSTRACT_PUBMED_19744497}}
 
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==About this Structure==
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==Crystal structure of a ternary complex of aklavinone-11 hydroxylase with FAD and aklavinone==
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[[3ihg]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IHG OCA].
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<StructureSection load='3ihg' size='340' side='right'caption='[[3ihg]], [[Resolution|resolution]] 2.49&Aring;' scene=''>
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== Structural highlights ==
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==Reference==
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<table><tr><td colspan='2'>[[3ihg]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_purpurascens Streptomyces purpurascens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IHG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IHG FirstGlance]. <br>
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<ref group="xtra">PMID:019744497</ref><references group="xtra"/>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.49&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=VAK:METHYL+(1R,2R,4S)-2-ETHYL-2,4,5,7-TETRAHYDROXY-6,11-DIOXO-1,2,3,4,6,11-HEXAHYDROTETRACENE-1-CARBOXYLATE'>VAK</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ihg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ihg OCA], [https://pdbe.org/3ihg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ihg RCSB], [https://www.ebi.ac.uk/pdbsum/3ihg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ihg ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/DNRF_STREF DNRF_STREF] Involved in the biosynthesis of the anthracyclines carminomycin, rhodomycin and daunorubicin (daunomycin) which are aromatic polyketide antibiotics that exhibit high cytotoxicity and are widely applied in the chemotherapy of a variety of cancers. Catalyzes the incorporation of a hydroxyl group at position C-11 of aklavinone, resulting in epsilon-rhodomycinone. It cannot accept substrates glycosylated at position C-7 and is specific for the C-9R configuration of anthracyclines. It can use both NAD or NADP but it is slowly inactivated in the presence of NADH.<ref>PMID:10082933</ref> <ref>PMID:19744497</ref>
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== Evolutionary Conservation ==
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[[Image:Consurf_key_small.gif|200px|right]]
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Check<jmol>
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<jmolCheckbox>
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ih/3ihg_consurf.spt"</scriptWhenChecked>
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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</jmolCheckbox>
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3ihg ConSurf].
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<div style="clear:both"></div>
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces purpurascens]]
[[Category: Streptomyces purpurascens]]
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[[Category: Jansson, A.]]
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[[Category: Jansson A]]
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[[Category: Koskiniemi, H.]]
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[[Category: Koskiniemi H]]
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[[Category: Lindqvist, Y.]]
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[[Category: Lindqvist Y]]
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[[Category: Sandalova, T.]]
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[[Category: Sandalova T]]
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[[Category: Schneider, G.]]
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[[Category: Schneider G]]
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[[Category: Anthracycline]]
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[[Category: Enzyme mechanism]]
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[[Category: Flavoenzyme]]
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[[Category: Flavoprotein]]
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[[Category: Hydroxylase]]
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[[Category: Merohedral twinning]]
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[[Category: Oxidoreductase]]
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[[Category: Polyketide biosynthesis]]
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Current revision

Crystal structure of a ternary complex of aklavinone-11 hydroxylase with FAD and aklavinone

PDB ID 3ihg

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