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3iya

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==Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH==
==Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH==
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<StructureSection load='3iya' size='340' side='right' caption='[[3iya]], [[Resolution|resolution]] 22.00&Aring;' scene=''>
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<SX load='3iya' size='340' side='right' viewer='molstar' caption='[[3iya]], [[Resolution|resolution]] 22.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3iya]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Aedes_albopictus Aedes albopictus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IYA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3IYA FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3iya]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Dengue_virus_2 Dengue virus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3IYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3IYA FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3iya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iya OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3iya RCSB], [http://www.ebi.ac.uk/pdbsum/3iya PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 22&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3iya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3iya OCA], [https://pdbe.org/3iya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3iya RCSB], [https://www.ebi.ac.uk/pdbsum/3iya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3iya ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/E7FLK7_9FLAV E7FLK7_9FLAV]
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Flavivirus assembles into an inert particle that requires proteolytic activation by furin to enable transmission to other hosts. We previously showed that immature virus undergoes a conformational change at low pH that renders it accessible to furin (I. M. Yu, W. Zhang, H. A. Holdaway, L. Li, V. A. Kostyuchenko, P. R. Chipman, R. J. Kuhn, M. G. Rossmann, and J. Chen, Science 319:1834-1837, 2008). Here we show, using cryoelectron microscopy, that the structure of immature dengue virus at pH 6.0 is essentially the same before and after the cleavage of prM. The structure shows that after cleavage, the proteolytic product pr remains associated with the virion at acidic pH, and that furin cleavage by itself does not induce any major conformational changes. We also show by liposome cofloatation experiments that pr retention prevents membrane insertion, suggesting that pr is present on the virion in the trans-Golgi network to protect the progeny virus from fusion within the host cell.
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Association of the pr peptides with dengue virus at acidic pH blocks membrane fusion.,Yu IM, Holdaway HA, Chipman PR, Kuhn RJ, Rossmann MG, Chen J J Virol. 2009 Dec;83(23):12101-7. Epub 2009 Sep 16. PMID:19759134<ref>PMID:19759134</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Aedes albopictus]]
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[[Category: Dengue virus 2]]
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[[Category: Chen, J]]
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[[Category: Large Structures]]
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[[Category: Chipman, P R]]
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[[Category: Chen J]]
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[[Category: Holdaway, H A]]
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[[Category: Chipman PR]]
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[[Category: Kuhn, R J]]
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[[Category: Holdaway HA]]
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[[Category: Rossmann, M G]]
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[[Category: Kuhn RJ]]
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[[Category: Yu, I]]
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[[Category: Rossmann MG]]
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[[Category: Icosahedral virus]]
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[[Category: Yu I]]
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[[Category: Prm]]
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[[Category: Virus]]
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Current revision

Association of the pr peptides with dengue virus blocks membrane fusion at acidic pH

3iya, resolution 22.00Å

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