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3j4u

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==A new topology of the HK97-like fold revealed in Bordetella bacteriophage: non-covalent chainmail secured by jellyrolls==
==A new topology of the HK97-like fold revealed in Bordetella bacteriophage: non-covalent chainmail secured by jellyrolls==
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<StructureSection load='3j4u' size='340' side='right' caption='[[3j4u]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
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<SX load='3j4u' size='340' side='right' viewer='molstar' caption='[[3j4u]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3j4u]] is a 14 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpbpp Bpbpp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3J4U FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3j4u]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_virus_BPP1 Bordetella virus BPP1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J4U FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3j4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3j4u RCSB], [http://www.ebi.ac.uk/pdbsum/3j4u PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j4u OCA], [https://pdbe.org/3j4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j4u RCSB], [https://www.ebi.ac.uk/pdbsum/3j4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j4u ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q775C7_BPBPP Q775C7_BPBPP]
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Bacteriophage BPP-1 infects and kills Bordetella species that cause whooping cough. Its diversity-generating retroelement (DGR) provides a naturally occurring phage-display system, but engineering efforts are hampered without atomic structures. Here, we report a cryo electron microscopy structure of the BPP-1 head at 3.5 A resolution. Our atomic model shows two of the three protein folds representing major viral lineages: jellyroll for its cement protein (CP) and HK97-like ('Johnson') for its major capsid protein (MCP). Strikingly, the fold topology of MCP is permuted non-circularly from the Johnson fold topology previously seen in viral and cellular proteins. We illustrate that the new topology is likely the only feasible alternative of the old topology. beta-sheet augmentation and electrostatic interactions contribute to the formation of non-covalent chainmail in BPP-1, unlike covalent inter-protein linkages of the HK97 chainmail. Despite these complex interactions, the termini of both CP and MCP are ideally positioned for DGR-based phage-display engineering. DOI: http://dx.doi.org/10.7554/eLife.01299.001.
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A new topology of the HK97-like fold revealed in Bordetella bacteriophage by cryoEM at 3.5 A resolution.,Zhang X, Guo H, Jin L, Czornyj E, Hodes A, Hui WH, Nieh AW, Miller JF, Zhou ZH Elife. 2013 Dec 17;2(0):e01299. doi: 10.7554/eLife.01299. PMID:24347545<ref>PMID:24347545</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Bpbpp]]
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[[Category: Bordetella virus BPP1]]
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[[Category: Czornyj, E]]
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[[Category: Large Structures]]
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[[Category: Guo, H]]
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[[Category: Czornyj E]]
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[[Category: Hodes, A]]
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[[Category: Guo H]]
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[[Category: Hui, W H]]
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[[Category: Hodes A]]
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[[Category: Jin, L]]
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[[Category: Hui WH]]
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[[Category: Miller, J F]]
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[[Category: Jin L]]
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[[Category: Nieh, A W]]
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[[Category: Miller JF]]
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[[Category: Zhang, X]]
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[[Category: Nieh AW]]
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[[Category: Zhou, Z H]]
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[[Category: Zhang X]]
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[[Category: Cryoem]]
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[[Category: Zhou ZH]]
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[[Category: Protein topology]]
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[[Category: Virus]]
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Current revision

A new topology of the HK97-like fold revealed in Bordetella bacteriophage: non-covalent chainmail secured by jellyrolls

3j4u, resolution 3.50Å

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