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3ooc

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==Crystal structure of the membrane fusion protein CusB from Escherichia coli==
==Crystal structure of the membrane fusion protein CusB from Escherichia coli==
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<StructureSection load='3ooc' size='340' side='right' caption='[[3ooc]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
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<StructureSection load='3ooc' size='340' side='right'caption='[[3ooc]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3ooc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3h9i 3h9i]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OOC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OOC FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3ooc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3h9i 3h9i]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OOC FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3opo|3opo]], [[3ow7|3ow7]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.404&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cusB, ylcD, b0574, JW0563 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ooc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ooc OCA], [https://pdbe.org/3ooc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ooc RCSB], [https://www.ebi.ac.uk/pdbsum/3ooc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ooc ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ooc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ooc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3ooc RCSB], [http://www.ebi.ac.uk/pdbsum/3ooc PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/CUSB_ECOLI CUSB_ECOLI] Part of a cation efflux system that mediates resistance to copper and silver.<ref>PMID:11399769</ref> <ref>PMID:12813074</ref>
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Gram-negative bacteria, such as Escherichia coli, frequently utilize tripartite efflux complexes belonging to the resistance-nodulation-division family to expel diverse toxic compounds from the cell. These systems contain a periplasmic membrane fusion protein (MFP) that is critical for substrate transport. We here present the x-ray structures of the CusB MFP from the copper/silver efflux system of E. coli. This is the first structure of any MFPs associated with heavy-metal efflux transporters. CusB bridges the inner-membrane efflux pump CusA and outer-membrane channel CusC to mediate resistance to Cu(+) and Ag(+) ions. Two distinct structures of the elongated molecules of CusB were found in the asymmetric unit of a single crystal, which suggests the flexible nature of this protein. Each protomer of CusB can be divided into four different domains, whereby the first three domains are mostly beta-strands and the last domain adopts an entirely helical architecture. Unlike other known structures of MFPs, the alpha-helical domain of CusB is folded into a three-helix bundle. This three-helix bundle presumably interacts with the periplasmic domain of CusC. The N- and C-termini of CusB form the first beta-strand domain, which is found to interact with the periplasmic domain of the CusA efflux pump. Atomic details of how this efflux protein binds Cu(+) and Ag(+) were revealed by the crystals of the CusB-Cu(I) and CusB-Ag(I) complexes. The structures indicate that CusB consists of multiple binding sites for these metal ions. These findings reveal novel structural features of an MFP in the resistance-nodulation-division efflux system and provide direct evidence that this protein specifically interacts with transported substrates.
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Crystal structure of the membrane fusion protein CusB from Escherichia coli.,Su CC, Yang F, Long F, Reyon D, Routh MD, Kuo DW, Mokhtari AK, Van Ornam JD, Rabe KL, Hoy JA, Lee YJ, Rajashankar KR, Yu EW J Mol Biol. 2009 Oct 23;393(2):342-55. Epub 2009 Aug 18. PMID:19695261<ref>PMID:19695261</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Escherichia coli K-12]]
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[[Category: Hoy, J A]]
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[[Category: Large Structures]]
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[[Category: Kuo, D W]]
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[[Category: Hoy JA]]
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[[Category: Lee, Y J]]
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[[Category: Kuo DW]]
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[[Category: Long, F]]
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[[Category: Lee YJ]]
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[[Category: Mokhtari, A K]]
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[[Category: Long F]]
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[[Category: Ornam, J D.Van]]
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[[Category: Mokhtari AK]]
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[[Category: Rabe, K L]]
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[[Category: Rabe KL]]
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[[Category: Rajashankar, K R]]
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[[Category: Rajashankar KR]]
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[[Category: Reyon, D]]
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[[Category: Reyon D]]
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[[Category: Routh, M D]]
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[[Category: Routh MD]]
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[[Category: Su, C C]]
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[[Category: Su C-C]]
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[[Category: Yang, F]]
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[[Category: Van Ornam JD]]
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[[Category: Yu, E W]]
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[[Category: Yang F]]
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[[Category: Beta barrel]]
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[[Category: Yu EW]]
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[[Category: Copper transport]]
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[[Category: Cusa]]
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[[Category: Cusc]]
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[[Category: Ion transport]]
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[[Category: Three-helix bundle]]
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[[Category: Transport]]
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[[Category: Transport protein]]
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Current revision

Crystal structure of the membrane fusion protein CusB from Escherichia coli

PDB ID 3ooc

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