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3ova

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How the CCA-adding Enzyme Selects Adenine over Cytosine in Position 76 of tRNA

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Retrieved from "http://52.214.119.220/wiki/index.php/3ova"

Categories: Archaeoglobus fulgidus | Large Structures | Pan BC | Steitz TA | Xiong Y

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 ==How the CCA-adding Enzyme Selects Adenine over Cytosine in Position 76 of tRNA==
-<StructureSection load='3ova' size='340' side='right' caption='[[3ova]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
+<StructureSection load='3ova' size='340' side='right'caption='[[3ova]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ova]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3OVA FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ova]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3OVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3OVA FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.98&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ouy|3ouy]], [[3ov7|3ov7]], [[3ovb|3ovb]], [[3ovs|3ovs]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APC:DIPHOSPHOMETHYLPHOSPHONIC+ACID+ADENOSYL+ESTER'>APC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ova FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ova OCA], [http://pdbe.org/3ova PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ova RCSB], [http://www.ebi.ac.uk/pdbsum/3ova PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ova ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ova FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ova OCA], [https://pdbe.org/3ova PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ova RCSB], [https://www.ebi.ac.uk/pdbsum/3ova PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ova ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/CCA_ARCFU CCA_ARCFU]] Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.<ref>PMID:14592988</ref>
+[https://www.uniprot.org/uniprot/CCA_ARCFU CCA_ARCFU] Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.<ref>PMID:14592988</ref>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-CCA-adding enzymes [ATP(CTP):tRNA nucleotidyltransferases] add CCA onto the 3' end of transfer RNA (tRNA) precursors without using a nucleic acid template. Although the mechanism by which cytosine (C) is selected at position 75 of tRNA has been established, the mechanism by which adenine (A) is selected at position 76 remains elusive. Here, we report five cocrystal structures of the enzyme complexed with both a tRNA mimic and nucleoside triphosphates under catalytically active conditions. These structures suggest that adenosine 5'-monophosphate is incorporated onto the A76 position of the tRNA via a carboxylate-assisted, one-metal-ion mechanism with aspartate 110 functioning as a general base. The discrimination against incorporation of cytidine 5'-triphosphate (CTP) at position 76 arises from improper placement of the alpha phosphate of the incoming CTP, which results from the interaction of C with arginine 224 and prevents the nucleophilic attack by the 3' hydroxyl group of cytidine75.
-How the CCA-adding enzyme selects adenine over cytosine at position 76 of tRNA.,Pan B, Xiong Y, Steitz TA Science. 2010 Nov 12;330(6006):937-40. PMID:21071662<ref>PMID:21071662</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 3ova" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[CCA-adding enzyme|CCA-adding enzyme]]
+*[[CCA-adding enzyme 3D structures|CCA-adding enzyme 3D structures]]
-*[[TRNA|TRNA]]
+*[[Transfer RNA (tRNA)|Transfer RNA (tRNA)]]
 == References ==
 <references/>
@@ Line 28: / Line 19: @@
 </StructureSection>
 [[Category: Archaeoglobus fulgidus]]
-[[Category: Pan, B C]]
+[[Category: Large Structures]]
-[[Category: Steitz, T A]]
+[[Category: Pan BC]]
-[[Category: Xiong, Y]]
+[[Category: Steitz TA]]
-[[Category: Cca-adding]]
+[[Category: Xiong Y]]
-[[Category: Nucleotidyltransfer]]
-[[Category: Protein-rna]]
-[[Category: Rossmann fold]]
-[[Category: Transferase-rna complex]]
-[[Category: Trna]]

3ova

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How the CCA-adding Enzyme Selects Adenine over Cytosine in Position 76 of tRNA

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