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3p74

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==H181N mutant of pentaerythritol tetranitrate reductase containing a C-terminal His8-tag==
==H181N mutant of pentaerythritol tetranitrate reductase containing a C-terminal His8-tag==
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<StructureSection load='3p74' size='340' side='right' caption='[[3p74]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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<StructureSection load='3p74' size='340' side='right'caption='[[3p74]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3p74]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter cloacae" (jordan 1890) bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P74 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P74 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3p74]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P74 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P74 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1h50|1h50]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">onr, PETNR ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p74 OCA], [https://pdbe.org/3p74 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p74 RCSB], [https://www.ebi.ac.uk/pdbsum/3p74 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p74 ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/NADPH_dehydrogenase NADPH dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.6.99.1 1.6.99.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p74 OCA], [http://pdbe.org/3p74 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3p74 RCSB], [http://www.ebi.ac.uk/pdbsum/3p74 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3p74 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/P71278_ENTCL P71278_ENTCL]
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We have conducted a site-specific saturation mutagenesis study of H181 and H184 of flavoprotein pentaerythritol tetranitrate reductase (PETN reductase) to probe the role of these residues in substrate binding and catalysis with a variety of alpha,beta-unsaturated alkenes. Single mutations at these residues were sufficient to dramatically increase the enantiopurity of products formed by reduction of 2-phenyl-1-nitropropene. In addition, many mutants exhibited a switch in reactivity to predominantly catalyse nitro reduction, as opposed to CC reduction. These mutants showed an enhancement in a minor side reaction and formed 2-phenylpropanal oxime from 2-phenyl-1-nitropropene. The multiple binding conformations of hydroxy substituted nitro-olefins in PETN reductase were examined by using both structural and catalytic techniques. These compounds were found to bind in both active and inhibitory complexes; this highlights the plasticity of the active site and the ability of the H181/H184 couple to coordinate with multiple functional groups. These properties demonstrate the potential to use PETN reductase as a scaffold in the development of industrially useful biocatalysts.
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A Site-Saturated Mutagenesis Study of Pentaerythritol Tetranitrate Reductase Reveals that Residues 181 and 184 Influence Ligand Binding, Stereochemistry and Reactivity.,Toogood HS, Fryszkowska A, Hulley M, Sakuma M, Mansell D, Stephens GM, Gardiner JM, Scrutton NS Chembiochem. 2011 Mar 21;12(5):738-49. doi: 10.1002/cbic.201000662. Epub, 2011 Mar 4. PMID:21374779<ref>PMID:21374779</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3p74" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
*[[Pentaerythritol tetranitrate reductase|Pentaerythritol tetranitrate reductase]]
*[[Pentaerythritol tetranitrate reductase|Pentaerythritol tetranitrate reductase]]
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== References ==
 
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<references/>
 
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: NADPH dehydrogenase]]
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[[Category: Enterobacter cloacae]]
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[[Category: Scrutton, N S]]
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[[Category: Large Structures]]
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[[Category: Toogood, H S]]
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[[Category: Scrutton NS]]
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[[Category: Alpha/beta barrel]]
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[[Category: Toogood HS]]
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[[Category: Old Yellow Enzyme]]
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[[Category: Oxidoreductase]]
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Current revision

H181N mutant of pentaerythritol tetranitrate reductase containing a C-terminal His8-tag

PDB ID 3p74

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