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3pgw

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==Crystal structure of human U1 snRNP==
==Crystal structure of human U1 snRNP==
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<StructureSection load='3pgw' size='340' side='right' caption='[[3pgw]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
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<StructureSection load='3pgw' size='340' side='right'caption='[[3pgw]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3pgw]] is a 22 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PGW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3PGW FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3pgw]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PGW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PGW FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3pgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pgw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3pgw RCSB], [http://www.ebi.ac.uk/pdbsum/3pgw PDBsum]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.4&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pgw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pgw OCA], [https://pdbe.org/3pgw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pgw RCSB], [https://www.ebi.ac.uk/pdbsum/3pgw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pgw ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/SNRPA_HUMAN SNRPA_HUMAN] Binds stem loop II of U1 snRNA. It is the first snRNP to interact with pre-mRNA. This interaction is required for the subsequent binding of U2 snRNP and the U4/U6/U5 tri-snRNP. In a snRNP-free form (SF-A) may be involved in coupled pre-mRNA splicing and polyadenylation process. Binds preferentially to the 5'-UGCAC-3' motif in vitro.<ref>PMID:9848648</ref>
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U1 small nuclear ribonucleoprotein (snRNP) recognizes the 5'-splice site early during spliceosome assembly. It represents a prototype spliceosomal subunit containing a paradigmatic Sm core RNP. The crystal structure of human U1 snRNP obtained from natively purified material by in situ limited proteolysis at 4.4 A resolution reveals how the seven Sm proteins, each recognize one nucleotide of the Sm site RNA using their Sm1 and Sm2 motifs. Proteins D1 and D2 guide the snRNA into and out of the Sm ring, and proteins F and E mediate a direct interaction between the Sm site termini. Terminal extensions of proteins D1, D2 and B/B', and extended internal loops in D2 and B/B' support a four-way RNA junction and a 3'-terminal stem-loop on opposite sides of the Sm core RNP, respectively. On a higher organizational level, the core RNP presents multiple attachment sites for the U1-specific 70K protein. The intricate, multi-layered interplay of proteins and RNA rationalizes the hierarchical assembly of U snRNPs in vitro and in vivo.
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Functional organization of the Sm core in the crystal structure of human U1 snRNP.,Weber G, Trowitzsch S, Kastner B, Luhrmann R, Wahl MC EMBO J. 2010 Dec 15;29(24):4172-84. Epub 2010 Nov 26. PMID:21113136<ref>PMID:21113136</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Lsm|Lsm]]
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*[[Nucleoprotein 3D structures|Nucleoprotein 3D structures]]
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*[[Nucleoprotein|Nucleoprotein]]
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*[[Sm-like protein 3D structures|Sm-like protein 3D structures]]
== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
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[[Category: Kastner, B]]
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[[Category: Large Structures]]
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[[Category: Luehrmann, R]]
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[[Category: Kastner B]]
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[[Category: Trowitzsch, S]]
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[[Category: Luehrmann R]]
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[[Category: Wahl, M C]]
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[[Category: Trowitzsch S]]
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[[Category: Weber, G]]
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[[Category: Wahl MC]]
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[[Category: Mrna]]
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[[Category: Weber G]]
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[[Category: Protein-rna complex]]
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[[Category: Rrm]]
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[[Category: Sm core]]
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[[Category: Sm fold]]
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[[Category: Snrnp]]
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[[Category: Splicing]]
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[[Category: Splicing factor]]
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[[Category: Splicing-dna-rna complex]]
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[[Category: U1 snrna]]
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Current revision

Crystal structure of human U1 snRNP

PDB ID 3pgw

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