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| | <StructureSection load='3pmd' size='340' side='right'caption='[[3pmd]], [[Resolution|resolution]] 1.76Å' scene=''> | | <StructureSection load='3pmd' size='340' side='right'caption='[[3pmd]], [[Resolution|resolution]] 1.76Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3pmd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_cereus_var._anthracis"_(cohn_1872)_smith_et_al._1946 "bacillus cereus var. anthracis" (cohn 1872) smith et al. 1946]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PMD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3pmd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis Bacillus anthracis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3PMD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3PMD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=11A:UNDECANOIC+ACID'>11A</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[3pmc|3pmc]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=11A:UNDECANOIC+ACID'>11A</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">GBAA_pXO1_0148, pX01-118 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1392 "Bacillus cereus var. anthracis" (Cohn 1872) Smith et al. 1946])</td></tr>
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| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pmd OCA], [https://pdbe.org/3pmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pmd RCSB], [https://www.ebi.ac.uk/pdbsum/3pmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pmd ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3pmd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3pmd OCA], [https://pdbe.org/3pmd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3pmd RCSB], [https://www.ebi.ac.uk/pdbsum/3pmd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3pmd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q44635_BACAN Q44635_BACAN] |
| - | Pathogenesis by Bacillus anthracis requires coordination between two distinct activities: plasmid-encoded virulence factor expression (which protects vegetative cells from immune surveillance during outgrowth and replication) and chromosomally encoded sporulation (required only during the final stages of infection). Sporulation is regulated by at least five sensor histidine kinases that are activated in response to various environmental cues. One of these kinases, BA2291, harbors a sensor domain that has approximately 35% sequence identity with two plasmid proteins, pXO1-118 and pXO2-61. Because overexpression of pXO2-61 (or pXO1-118) inhibits sporulation of B. anthracis in a BA2291-dependent manner, and pXO2-61 expression is strongly up-regulated by the major virulence gene regulator, AtxA, it was suggested that their function is to titrate out an environmental signal that would otherwise promote untimely sporulation. To explore this hypothesis, we determined crystal structures of both plasmid-encoded proteins. We found that they adopt a dimeric globin fold but, most unusually, do not bind heme. Instead, they house a hydrophobic tunnel and hydrophilic chamber that are occupied by fatty acid, which engages a conserved arginine and chloride ion via its carboxyl head group. In vivo, these domains may therefore recognize changes in fatty acid synthesis, chloride ion concentration, and/or pH. Structure-based comparisons with BA2291 suggest that it binds ligand and dimerizes in an analogous fashion, consistent with the titration hypothesis. Analysis of newly sequenced bacterial genomes points to the existence of a much broader family of non-heme, globin-based sensor domains, with related but distinct functionalities, that may have evolved from an ancestral heme-linked globin.
| + | |
| - | | + | |
| - | Structural Insights into Inhibition of Bacillus anthracis Sporulation by a Novel Class of Non-heme Globin Sensor Domains.,Stranzl GR, Santelli E, Bankston LA, La Clair C, Bobkov A, Schwarzenbacher R, Godzik A, Perego M, Grynberg M, Liddington RC J Biol Chem. 2011 Mar 11;286(10):8448-58. Epub 2011 Jan 7. PMID:21216948<ref>PMID:21216948</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 3pmd" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Large Structures]] | |
| - | [[Category: Bankston, L A]] | |
| - | [[Category: Bobkov, A]] | |
| - | [[Category: Clair, C La]] | |
| - | [[Category: Godzik, A]] | |
| - | [[Category: Grynberg, M]] | |
| - | [[Category: Liddington, R C]] | |
| - | [[Category: Perego, M]] | |
| - | [[Category: Santelli, E]] | |
| - | [[Category: Schwarzenbacher, R]] | |
| - | [[Category: Stranzl, G R]] | |
| | [[Category: Bacillus anthracis]] | | [[Category: Bacillus anthracis]] |
| - | [[Category: Chloride coordination]] | + | [[Category: Large Structures]] |
| - | [[Category: Fatty acid-binding protein]] | + | [[Category: Bankston LA]] |
| - | [[Category: Globin fold]] | + | [[Category: Bobkov A]] |
| - | [[Category: Kinase sensor domain]] | + | [[Category: Godzik A]] |
| - | [[Category: Lipid binding protein]] | + | [[Category: Grynberg M]] |
| - | [[Category: Non-heme globin]] | + | [[Category: La Clair C]] |
| - | [[Category: Sporulation]] | + | [[Category: Liddington RC]] |
| | + | [[Category: Perego M]] |
| | + | [[Category: Santelli E]] |
| | + | [[Category: Schwarzenbacher R]] |
| | + | [[Category: Stranzl GR]] |
