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3qva

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==Structure of Klebsiella pneumoniae 5-hydroxyisourate hydrolase==
==Structure of Klebsiella pneumoniae 5-hydroxyisourate hydrolase==
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<StructureSection load='3qva' size='340' side='right' caption='[[3qva]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
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<StructureSection load='3qva' size='340' side='right'caption='[[3qva]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3qva]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Klep7 Klep7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QVA FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3qva]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae_subsp._pneumoniae_MGH_78578 Klebsiella pneumoniae subsp. pneumoniae MGH 78578]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QVA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QVA FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.755&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HpxT, KPN78578_16360, KPN_01666 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272620 KLEP7])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qva OCA], [http://pdbe.org/3qva PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3qva RCSB], [http://www.ebi.ac.uk/pdbsum/3qva PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3qva ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qva FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qva OCA], [https://pdbe.org/3qva PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qva RCSB], [https://www.ebi.ac.uk/pdbsum/3qva PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qva ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A6T926_KLEP7 A6T926_KLEP7]
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The stereospecific oxidative degradation of uric acid to (S)-allantoin has recently been demonstrated to proceed via two unstable intermediates and requires three separate enzymatic reactions. The second step of this reaction, the conversion of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline, is catalyzed by HIU hydrolase (HIUH). The high-resolution crystal structure of HIUH from the opportunistic pathogen Klebsiella pneumoniae (KpHIUH) has been determined. KpHIUH is a homotetrameric protein that, based on sequence and structural similarity, belongs to the transthyretin-related protein family. In addition, the steady-state kinetic parameters for this enzyme and four active-site mutants have been measured. These data provide valuable insight into the functional roles of the active-site residues. Based upon the structural and kinetic data, a mechanism is proposed for the KpHIUH-catalyzed reaction.
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Structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from Klebsiella pneumoniae.,French JB, Ealick SE Acta Crystallogr D Biol Crystallogr. 2011 Aug;67(Pt 8):671-7. Epub 2011, Jul 12. PMID:21795808<ref>PMID:21795808</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3qva" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Klep7]]
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[[Category: Klebsiella pneumoniae subsp. pneumoniae MGH 78578]]
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[[Category: Ealick, S E]]
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[[Category: Large Structures]]
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[[Category: French, J B]]
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[[Category: Ealick SE]]
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[[Category: 5-hydroxyisourate hydrolase]]
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[[Category: French JB]]
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[[Category: Hydrolase]]
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[[Category: Transthyretin-related protein]]
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Current revision

Structure of Klebsiella pneumoniae 5-hydroxyisourate hydrolase

PDB ID 3qva

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