This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


3qy2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:50, 21 February 2024) (edit) (undo)
 
(2 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of the P93A monomer mutant of S. cerevisiae Cks1==
==Crystal structure of the P93A monomer mutant of S. cerevisiae Cks1==
-
<StructureSection load='3qy2' size='340' side='right' caption='[[3qy2]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
+
<StructureSection load='3qy2' size='340' side='right'caption='[[3qy2]], [[Resolution|resolution]] 2.59&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3qy2]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3QY2 FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3qy2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3QY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3QY2 FirstGlance]. <br>
-
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.59&#8491;</td></tr>
-
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CKS1, YBR135W, YBR1011 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3qy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qy2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3qy2 RCSB], [http://www.ebi.ac.uk/pdbsum/3qy2 PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3qy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3qy2 OCA], [https://pdbe.org/3qy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3qy2 RCSB], [https://www.ebi.ac.uk/pdbsum/3qy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3qy2 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
-
[[http://www.uniprot.org/uniprot/CKS1_YEAST CKS1_YEAST]] Binds to the catalytic subunit of the cyclin dependent kinase (CDC28) and is essential for its biological function.
+
[https://www.uniprot.org/uniprot/CKS1_YEAST CKS1_YEAST] Binds to the catalytic subunit of the cyclin dependent kinase (CDC28) and is essential for its biological function.
-
<div style="background-color:#fffaf0;">
+
-
== Publication Abstract from PubMed ==
+
-
Cks (cyclin-dependent kinase subunit) proteins are essential eukaryotic cell cycle regulatory proteins that physically associate with cyclin-dependent kinases (Cdks) to modulate their activity. Cks proteins have also been studied for their ability to form domain-swapped dimers by exchanging beta-strands. Domain swapping is mediated by a conserved beta-hinge region containing two proline residues. Previous structural studies indicate that Cks in its dimer form is unable to bind Cdk, suggesting that the monomer-dimer equilibrium of Cks may have an effect on Cks-mediated Cdk regulation. We present the crystal structure of a proline-to-alanine mutant Saccharomyces cerevisiae Cks protein (Cks1 P93A) that preferentially adopts the monomer conformation but surprisingly fails to bind Cdk. Comparison of the Cks1 P93A structure to that of other Cks proteins reveals that Pro93 is critical for stabilizing a multiple beta-turn structure in the hinge region that properly positions an essential Cdk-binding residue. Additionally, we find that these beta-turn formations, conserved in Cks homologs, have implications for the mechanism and preferentiality of strand exchange. Together, our observations suggest that the conservation of Cks hinge-region prolines reflects their functions in forming a Cdk binding interface and that the ability of these prolines to control partitioning between monomer and dimer is a consequence of the beta-turn networks within the hinge.
+
-
 
+
-
The Structure of a Monomeric Mutant Cks Protein Reveals Multiple Functions for a Conserved Hinge-Region Proline.,Balog ER, Saetern OC, Finch W, Hoeft CO, Thai V, Harvey SL, Kellogg DR, Rubin SM J Mol Biol. 2011 Jun 17. PMID:21704044<ref>PMID:21704044</ref>
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
==See Also==
-
</div>
+
*[[Cyclin-dependent kinase regulatory subunit 3D structures|Cyclin-dependent kinase regulatory subunit 3D structures]]
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
 +
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
-
[[Category: Balog, E R.M]]
+
[[Category: Balog ERM]]
-
[[Category: Finch, W]]
+
[[Category: Finch W]]
-
[[Category: Harvey, S L]]
+
[[Category: Harvey SL]]
-
[[Category: Hoeft, C O]]
+
[[Category: Hoeft CO]]
-
[[Category: Kellogg, D K]]
+
[[Category: Kellogg DK]]
-
[[Category: Rubin, S M]]
+
[[Category: Rubin SM]]
-
[[Category: Saetern, O C]]
+
[[Category: Saetern OC]]
-
[[Category: Thai, V]]
+
[[Category: Thai V]]
-
[[Category: Cdc28 kinase]]
+
-
[[Category: Cell cycle]]
+
-
[[Category: Cyclin-dependent kinase]]
+
-
[[Category: Protein kinase activator]]
+
-
[[Category: Transcription]]
+
-
[[Category: Transferase regulator]]
+
-
[[Category: Ubiquitin binding]]
+

Current revision

Crystal structure of the P93A monomer mutant of S. cerevisiae Cks1

PDB ID 3qy2

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3qy2"
Personal tools