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3r3p

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==Homing Endonuclease I-Bth0305I Catalytic Domain==
==Homing Endonuclease I-Bth0305I Catalytic Domain==
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<StructureSection load='3r3p' size='340' side='right' caption='[[3r3p]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
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<StructureSection load='3r3p' size='340' side='right'caption='[[3r3p]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3r3p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Viruses Viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R3P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R3P FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3r3p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_0305phi8-36 Bacillus phage 0305phi8-36]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R3P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R3P FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF029, 0305phi8-36p029 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10239 Viruses])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r3p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3r3p RCSB], [http://www.ebi.ac.uk/pdbsum/3r3p PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r3p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r3p OCA], [https://pdbe.org/3r3p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r3p RCSB], [https://www.ebi.ac.uk/pdbsum/3r3p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r3p ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A7KV39_9CAUD A7KV39_9CAUD]
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Novel family of putative homing endonuclease genes was recently discovered during analyses of metagenomic and genomic sequence data. One such protein is encoded within a group I intron that resides in the recA gene of the Bacillus thuringiensis 03058-36 bacteriophage. Named I-Bth0305I, the endonuclease cleaves a DNA target in the uninterrupted recA gene at a position immediately adjacent to the intron insertion site. The enzyme displays a multidomain, homodimeric architecture and footprints a DNA region of approximately 60 bp. Its highest specificity corresponds to a 14-bp pseudopalindromic sequence that is directly centered across the DNA cleavage site. Unlike many homing endonucleases, the specificity profile of the enzyme is evenly distributed across much of its target site, such that few single base pair substitutions cause a significant decrease in cleavage activity. A crystal structure of its C-terminal domain confirms a nuclease fold that is homologous to very short patch repair (Vsr) endonucleases. The domain architecture and DNA recognition profile displayed by I-Bth0305I, which is the prototype of a homing lineage that we term the 'EDxHD' family, are distinct from previously characterized homing endonucleases.
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Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family.,Taylor GK, Heiter DF, Pietrokovski S, Stoddard BL Nucleic Acids Res. 2011 Sep 2. PMID:21890897<ref>PMID:21890897</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Viruses]]
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[[Category: Bacillus phage 0305phi8-36]]
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[[Category: Stoddard, B L]]
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[[Category: Large Structures]]
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[[Category: Taylor, G K]]
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[[Category: Stoddard BL]]
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[[Category: Homing endonuclease]]
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[[Category: Taylor GK]]
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[[Category: Hydrolase]]
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Current revision

Homing Endonuclease I-Bth0305I Catalytic Domain

PDB ID 3r3p

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