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3p8f

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==Crystal Structure of MT-SP1 in complex with SFTI-1==
==Crystal Structure of MT-SP1 in complex with SFTI-1==
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<StructureSection load='3p8f' size='340' side='right' caption='[[3p8f]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='3p8f' size='340' side='right'caption='[[3p8f]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3p8f]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P8F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3P8F FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3p8f]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Helianthus_annuus Helianthus annuus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3P8F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3P8F FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3p8g|3p8g]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ST14 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3p8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p8f OCA], [https://pdbe.org/3p8f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3p8f RCSB], [https://www.ebi.ac.uk/pdbsum/3p8f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3p8f ProSAT]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Matriptase Matriptase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.109 3.4.21.109] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3p8f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3p8f OCA], [http://pdbe.org/3p8f PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3p8f RCSB], [http://www.ebi.ac.uk/pdbsum/3p8f PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3p8f ProSAT]</span></td></tr>
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</table>
</table>
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== Disease ==
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[https://www.uniprot.org/uniprot/ST14_HUMAN ST14_HUMAN] Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH) [MIM:[https://omim.org/entry/610765 610765]. ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair.<ref>PMID:17273967</ref>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/SFTI1_HELAN SFTI1_HELAN]] Inhibits trypsin, cathepsin G, elastase, chymotrypsin and thrombin. Does not inhibit factor Xa.<ref>PMID:10390350</ref>
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[https://www.uniprot.org/uniprot/ST14_HUMAN ST14_HUMAN] Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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ABSTRACT: BACKGROUND: Matriptase is a type II transmembrane serine protease that is found on the surfaces of epithelial cells and certain cancer cells. Matriptase has been implicated in the degradation of certain extracellular matrix components as well as the activation of various cellular proteins and proteases, including hepatocyte growth factor and urokinase. Sunflower trypsin inhibitor-1 (SFTI-1), a cyclic peptide inhibitor originally isolated from sunflower seeds, exhibits potent inhibitory activity toward matriptase. RESULTS: We have engineered and produced recombinant proteins of the matriptase protease domain, and have determined the crystal structures of the protease:SFTI-1 complex at 2.0 A as well as the protease:benzamidine complex at 1.2 A. These structures elaborate the structural basis of substrate selectivity of matriptase, and show that the matriptase S1 substrate specificity pocket is larger enough to allow movement of benzamidine inside the S1 pocket. Our study also reveals that SFTI-1 binds to matriptase in a way similar to its binding to trypsin despite the significantly different isoelectric points of the two proteins (5.6 vs. 8.2). CONCLUSIONS: This work helps to define the structural basis of substrate specificity of matriptase and the interactions between the inhibitor and protease. The complex structure also provides a structural template for designing new SFTI-1 derivatives with better potency and selectivity against matriptase and other proteases.
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Structure of catalytic domain of Matriptase in complex with Sunflower trypsin inhibitor-1.,Yuan C, Chen L, Meehan EJ, Daly N, Craik DJ, Huang M, Ngo JC BMC Struct Biol. 2011 Jun 22;11:30. PMID:21693064<ref>PMID:21693064</ref>
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==See Also==
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*[[Matriptase 3D structures|Matriptase 3D structures]]
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
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</div>
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<div class="pdbe-citations 3p8f" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Human]]
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[[Category: Helianthus annuus]]
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[[Category: Matriptase]]
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[[Category: Homo sapiens]]
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[[Category: Chen, L]]
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[[Category: Large Structures]]
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[[Category: Huang, M]]
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[[Category: Chen L]]
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[[Category: Yuan, C]]
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[[Category: Huang M]]
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[[Category: Hydrolase-hydrolase inhibitor complex]]
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[[Category: Yuan C]]
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[[Category: Protein-inhibitor complex]]
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Current revision

Crystal Structure of MT-SP1 in complex with SFTI-1

PDB ID 3p8f

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