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3rv0

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==Crystal structure of K. polysporus Dcr1 without the C-terminal dsRBD==
==Crystal structure of K. polysporus Dcr1 without the C-terminal dsRBD==
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<StructureSection load='3rv0' size='340' side='right' caption='[[3rv0]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
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<StructureSection load='3rv0' size='340' side='right'caption='[[3rv0]], [[Resolution|resolution]] 2.29&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3rv0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Kluyveromyces_polysporus Kluyveromyces polysporus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RV0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RV0 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3rv0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Vanderwaltozyma_polyspora_DSM_70294 Vanderwaltozyma polyspora DSM 70294]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RV0 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.29&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rv1|3rv1]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kpol_455p11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=436907 Kluyveromyces polysporus])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rv0 OCA], [https://pdbe.org/3rv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rv0 RCSB], [https://www.ebi.ac.uk/pdbsum/3rv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rv0 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rv0 OCA], [http://pdbe.org/3rv0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rv0 RCSB], [http://www.ebi.ac.uk/pdbsum/3rv0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rv0 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A7TR32_VANPO A7TR32_VANPO]
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The Dicer ribonuclease III (RNase III) enzymes process long double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs) that direct RNA interference. Here, we describe the structure and activity of a catalytically active fragment of Kluyveromyces polysporus Dcr1, which represents the noncanonical Dicers found in budding yeasts. The crystal structure revealed a homodimer resembling that of bacterial RNase III but extended by a unique N-terminal domain, and it identified additional catalytic residues conserved throughout eukaryotic RNase III enzymes. Biochemical analyses showed that Dcr1 dimers bind cooperatively along the dsRNA substrate such that the distance between consecutive active sites determines the length of the siRNA products. Thus, unlike canonical Dicers, which successively remove siRNA duplexes from the dsRNA termini, budding-yeast Dicers initiate processing in the interior and work outward. The distinct mechanism of budding-yeast Dicers establishes a paradigm for natural molecular rulers and imparts substrate preferences with ramifications for biological function.
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The inside-out mechanism of dicers from budding yeasts.,Weinberg DE, Nakanishi K, Patel DJ, Bartel DP Cell. 2011 Jul 22;146(2):262-76. PMID:21784247<ref>PMID:21784247</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3rv0" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Kluyveromyces polysporus]]
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[[Category: Large Structures]]
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[[Category: Bartel, D P]]
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[[Category: Vanderwaltozyma polyspora DSM 70294]]
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[[Category: Nakanishi, K]]
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[[Category: Bartel DP]]
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[[Category: Patel, D J]]
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[[Category: Nakanishi K]]
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[[Category: Weinberg, D E]]
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[[Category: Patel DJ]]
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[[Category: Rna binding protein]]
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[[Category: Weinberg DE]]
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[[Category: Rnase iii enzyme]]
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Current revision

Crystal structure of K. polysporus Dcr1 without the C-terminal dsRBD

PDB ID 3rv0

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