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3rv1

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==Crystal structure of the N-terminal and RNase III domains of K. polysporus Dcr1 E224Q mutant==
==Crystal structure of the N-terminal and RNase III domains of K. polysporus Dcr1 E224Q mutant==
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<StructureSection load='3rv1' size='340' side='right' caption='[[3rv1]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
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<StructureSection load='3rv1' size='340' side='right'caption='[[3rv1]], [[Resolution|resolution]] 1.98&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3rv1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vanderwaltozyma_polyspora Vanderwaltozyma polyspora]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RV1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RV1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3rv1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vanderwaltozyma_polyspora_DSM_70294 Vanderwaltozyma polyspora DSM 70294]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RV1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RV1 FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rv0|3rv0]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.975&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kpol_455p11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36033 Vanderwaltozyma polyspora])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rv1 OCA], [https://pdbe.org/3rv1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rv1 RCSB], [https://www.ebi.ac.uk/pdbsum/3rv1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rv1 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rv1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rv1 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rv1 RCSB], [http://www.ebi.ac.uk/pdbsum/3rv1 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/A7TR32_VANPO A7TR32_VANPO]
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The Dicer ribonuclease III (RNase III) enzymes process long double-stranded RNA (dsRNA) into small interfering RNAs (siRNAs) that direct RNA interference. Here, we describe the structure and activity of a catalytically active fragment of Kluyveromyces polysporus Dcr1, which represents the noncanonical Dicers found in budding yeasts. The crystal structure revealed a homodimer resembling that of bacterial RNase III but extended by a unique N-terminal domain, and it identified additional catalytic residues conserved throughout eukaryotic RNase III enzymes. Biochemical analyses showed that Dcr1 dimers bind cooperatively along the dsRNA substrate such that the distance between consecutive active sites determines the length of the siRNA products. Thus, unlike canonical Dicers, which successively remove siRNA duplexes from the dsRNA termini, budding-yeast Dicers initiate processing in the interior and work outward. The distinct mechanism of budding-yeast Dicers establishes a paradigm for natural molecular rulers and imparts substrate preferences with ramifications for biological function.
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The inside-out mechanism of dicers from budding yeasts.,Weinberg DE, Nakanishi K, Patel DJ, Bartel DP Cell. 2011 Jul 22;146(2):262-76. PMID:21784247<ref>PMID:21784247</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Vanderwaltozyma polyspora]]
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[[Category: Large Structures]]
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[[Category: Bartel, D P]]
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[[Category: Vanderwaltozyma polyspora DSM 70294]]
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[[Category: Nakanishi, K]]
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[[Category: Bartel DP]]
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[[Category: Patel, D J]]
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[[Category: Nakanishi K]]
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[[Category: Weinberg, D E]]
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[[Category: Patel DJ]]
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[[Category: Rna binding protein]]
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[[Category: Weinberg DE]]
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[[Category: Rnase iii enzyme]]
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Current revision

Crystal structure of the N-terminal and RNase III domains of K. polysporus Dcr1 E224Q mutant

PDB ID 3rv1

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