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3sbs

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==Crystal structure of Aar2 protein==
==Crystal structure of Aar2 protein==
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<StructureSection load='3sbs' size='340' side='right' caption='[[3sbs]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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<StructureSection load='3sbs' size='340' side='right'caption='[[3sbs]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3sbs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SBS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SBS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3sbs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SBS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SBS FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3sbg|3sbg]], [[3sbt|3sbt]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AAR2, YBL06.06, YBL0611, YBL074C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sbs OCA], [https://pdbe.org/3sbs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sbs RCSB], [https://www.ebi.ac.uk/pdbsum/3sbs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sbs ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sbs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sbs OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sbs RCSB], [http://www.ebi.ac.uk/pdbsum/3sbs PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/AAR2_YEAST AAR2_YEAST] Involved in splicing pre-mRNA of the A1 cistron and other genes that are important for cell growth.
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== Publication Abstract from PubMed ==
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Little is known about how particle-specific proteins are assembled on spliceosomal small nuclear ribonucleoproteins (snRNPs). Brr2p is a U5 snRNP-specific RNA helicase required for spliceosome catalytic activation and disassembly. In yeast, the Aar2 protein is part of a cytoplasmic precursor U5 snRNP that lacks Brr2p and is replaced by Brr2p in the nucleus. Here we show that Aar2p and Brr2p bind to different domains in the C-terminal region of Prp8p; Aar2p interacts with the RNaseH domain, whereas Brr2p interacts with the Jab1/MPN domain. These domains are connected by a long, flexible linker, but the Aar2p-RNaseH complex sequesters the Jab1/MPN domain, thereby preventing binding by Brr2p. Aar2p is phosphorylated in vivo, and a phospho-mimetic S253E mutation in Aar2p leads to disruption of the Aar2p-Prp8p complex in favor of the Brr2p-Prp8p complex. We propose a model in which Aar2p acts as a phosphorylation-controlled U5 snRNP assembly factor that regulates the incorporation of the particle-specific Brr2p. The purpose of this regulation may be to safeguard against nonspecific RNA binding to Prp8p and/or premature activation of Brr2p activity.
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Mechanism for Aar2p function as a U5 snRNP assembly factor.,Weber G, Cristao VF, Alves FD, Santos KF, Holton N, Rappsilber J, Beggs JD, Wahl MC Genes Dev. 2011 Jul 15. PMID:21764848<ref>PMID:21764848</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Saccharomyces cerevisiae]]
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[[Category: Large Structures]]
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[[Category: Holton, N.]]
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[[Category: Saccharomyces cerevisiae S288C]]
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[[Category: Santos, K F.]]
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[[Category: Holton N]]
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[[Category: Wahl, M C.]]
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[[Category: Santos KF]]
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[[Category: Weber, G.]]
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[[Category: Wahl MC]]
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[[Category: Splicing]]
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[[Category: Weber G]]
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[[Category: Vhs like domain]]
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Current revision

Crystal structure of Aar2 protein

PDB ID 3sbs

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