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3sh3

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==Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii STANDL==
==Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii STANDL==
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<StructureSection load='3sh3' size='340' side='right' caption='[[3sh3]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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<StructureSection load='3sh3' size='340' side='right'caption='[[3sh3]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3sh3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dioclea_wilsonii Dioclea wilsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SH3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SH3 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3sh3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Macropsychanthus_wilsonii Macropsychanthus wilsonii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SH3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3SH3 FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=A3B:D-VINYLGLYCINE'>A3B</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rrd|3rrd]], [[3rs6|3rs6]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A3B:D-VINYLGLYCINE'>A3B</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=XMM:(2R,3S,4S,5S,6R)-2-(5-BROMO-4-CHLORO-1H-INDOL-3-YLOXY)-TETRAHYDRO-6-(HYDROXYMETHYL)-2H-PYRAN-3,4,5-TRIOL'>XMM</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sh3 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3sh3 RCSB], [http://www.ebi.ac.uk/pdbsum/3sh3 PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3sh3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sh3 OCA], [https://pdbe.org/3sh3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3sh3 RCSB], [https://www.ebi.ac.uk/pdbsum/3sh3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3sh3 ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/LECA_MACWI LECA_MACWI] D-mannose/D-glucose-binding lectin with hemagglutinating activity towards rabbit and human erythrocytes. In rats, elicits an acute inflammatory response by inducing neutrophil migration and induces dose-dependent paw edema.<ref>PMID:21694673</ref> <ref>PMID:21924319</ref>
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The crystal structure and pro-inflammatory property of a lectin from the seeds of Dioclea wilsonii (DwL) were analyzed to gain a better understanding of structure/function relationships of Diocleinae lectins. Following crystallization and structural determination by standard molecular replacement techniques, DwL was found to be a tetramer based on PISA analysis, and composed by two metal-binding sites per monomer and loops which are involved in molecular oligomerization. DwL presents 96% and 99% identity with two other previously described lectins of Dioclearostrata (DRL) and Diocleagrandiflora (DGL). DwL differs structurally from DVL and DRL with regard to the conformation of the carbohydrate recognition domain and related biological activities. The structural analysis of DwL in comparison to other Diocleinae lectins can be related to the differences in the dose-dependent pro-inflammatory effect elicited in Wistar rats, probably via specific interactions with mast cells complex carbohydrate, resulting in significant paw edema. DwL appears to be involved in positive modulation of mast cell degranulation via recognition of surface carbohydrates. Since this recognition is dependent on site volume and CRD configuration, edematogenesis mediated by resident cells varies in potency and efficacy among different Diocleinae lectins.
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Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii Standl.,Rangel TB, Rocha BA, Bezerra GA, Assreuy AM, Pires AD, do Nascimento AS, Bezerra MJ, do Nascimento KS, Nagano CS, Sampaio AH, Gruber K, Delatorre P, Fernandes PM, Cavada BS Biochimie. 2011 Sep 10. PMID:21924319<ref>PMID:21924319</ref>
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Dioclea wilsonii]]
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[[Category: Large Structures]]
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[[Category: Assreuy, A M.S.]]
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[[Category: Macropsychanthus wilsonii]]
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[[Category: Bezerra, G A.]]
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[[Category: Assreuy AMS]]
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[[Category: Bezerra, M J.B.]]
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[[Category: Bezerra GA]]
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[[Category: Cavada, B S.]]
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[[Category: Bezerra MJB]]
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[[Category: Delatorre, P.]]
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[[Category: Cavada BS]]
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[[Category: Gruber, K.]]
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[[Category: Delatorre P]]
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[[Category: Nagano, C S.]]
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[[Category: Gruber K]]
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[[Category: Nascimento, K S.]]
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[[Category: Nagano CS]]
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[[Category: Rangel, T B.A.]]
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[[Category: Nascimento KS]]
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[[Category: Rocha, B A.M.]]
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[[Category: Rangel TBA]]
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[[Category: Sampaio, A H.]]
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[[Category: Rocha BAM]]
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[[Category: Carbohydrate binding protein]]
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[[Category: Sampaio AH]]
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[[Category: Jelly-roll]]
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[[Category: Legume lectin]]
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[[Category: Pro-inflammatory effect]]
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Current revision

Crystal structure of a pro-inflammatory lectin from the seeds of Dioclea wilsonii STANDL

PDB ID 3sh3

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