3tek

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==ThermoDBP: a non-canonical single-stranded DNA binding protein with a novel structure and mechanism==
==ThermoDBP: a non-canonical single-stranded DNA binding protein with a novel structure and mechanism==
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<StructureSection load='3tek' size='340' side='right' caption='[[3tek]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='3tek' size='340' side='right'caption='[[3tek]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[3tek]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35583 Atcc 35583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TEK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TEK FirstGlance]. <br>
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<table><tr><td colspan='2'>[[3tek]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TEK FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ttx1576 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2271 ATCC 35583])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tek OCA], [http://pdbe.org/3tek PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tek RCSB], [http://www.ebi.ac.uk/pdbsum/3tek PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tek ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tek OCA], [https://pdbe.org/3tek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tek RCSB], [https://www.ebi.ac.uk/pdbsum/3tek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tek ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/H0USY6_THETE H0USY6_THETE]
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ssDNA-binding proteins (SSBs) based on the oligonucleotide-binding fold are considered ubiquitous in nature and play a central role in many DNA transactions including replication, recombination, and repair. We demonstrate that the Thermoproteales, a clade of hyperthermophilic Crenarchaea, lack a canonical SSB. Instead, they encode a distinct ssDNA-binding protein that we term "ThermoDBP," exemplified by the protein Ttx1576 from Thermoproteus tenax. ThermoDBP binds specifically to ssDNA with low sequence specificity. The crystal structure of Ttx1576 reveals a unique fold and a mechanism for ssDNA binding, consisting of an extended cleft lined with hydrophobic phenylalanine residues and flanked by basic amino acids. Two ssDNA-binding domains are linked by a coiled-coil leucine zipper. ThermoDBP appears to have displaced the canonical SSB during the diversification of the Thermoproteales, a highly unusual example of the loss of a "ubiquitous" protein during evolution.
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Displacement of the canonical single-stranded DNA-binding protein in the Thermoproteales.,Paytubi S, McMahon SA, Graham S, Liu H, Botting CH, Makarova KS, Koonin EV, Naismith JH, White MF Proc Natl Acad Sci U S A. 2011 Nov 21. PMID:22106294<ref>PMID:22106294</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 3tek" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Single-stranded DNA-binding protein|Single-stranded DNA-binding protein]]
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*[[Single-stranded DNA-binding protein 3D structures|Single-stranded DNA-binding protein 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 35583]]
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[[Category: Large Structures]]
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[[Category: Graham, S]]
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[[Category: Thermoproteus tenax]]
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[[Category: Liu, H]]
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[[Category: Graham S]]
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[[Category: McMahon, S A]]
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[[Category: Liu H]]
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[[Category: Naismith, J H]]
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[[Category: McMahon SA]]
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[[Category: Paytubi, S]]
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[[Category: Naismith JH]]
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[[Category: White, M F]]
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[[Category: Paytubi S]]
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[[Category: Dna binding protein]]
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[[Category: White MF]]
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[[Category: Leucine zipper]]
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[[Category: Single stranded dna binding]]
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Current revision

ThermoDBP: a non-canonical single-stranded DNA binding protein with a novel structure and mechanism

PDB ID 3tek

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