1ql5
From Proteopedia
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'''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT''' | '''DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QL5 OCA]. | |
==Reference== | ==Reference== | ||
Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer., Lee JH, Choi YJ, Choi BS, Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10734199 10734199] | Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer., Lee JH, Choi YJ, Choi BS, Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10734199 10734199] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Choi, B S.]] | [[Category: Choi, B S.]] | ||
[[Category: Hwang, G S.]] | [[Category: Hwang, G S.]] | ||
[[Category: Lee, J H.]] | [[Category: Lee, J H.]] | ||
| - | [[Category: | + | [[Category: Deoxyribonucleic acid]] |
| - | + | [[Category: Dna photoproduct]] | |
| - | [[Category: | + | [[Category: Mutagenesis]] |
| - | [[Category: | + | [[Category: Nmr]] |
| - | [[Category: | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 06:24:20 2008'' |
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:24, 3 May 2008
DNA DECAMER DUPLEX CONTAINING T5-T6 PHOTOADDUCT
Overview
The cis - syn dimer is the major DNA photoproduct produced by UV irradiation. In order to determine the origin of the mutagenic property of the cis - syn dimer, we used NMR restraints and molecular dynamics to determine the solution structure of a DNA decamer duplex containing a wobble pair between the 3'-T of the cis - syn dimer and the opposite T residue (CS/TA duplex). The solution structure of the CS/TA duplex revealed that the 3'-T x T base pair of the cis - syn dimer had base pair geometry that was significantly different from the canonical Watson-Crick base pair and caused destabilization and conformational distortion of its 3'-region. However, a 3'-T x A base pair at the cis - syn dimer within this related DNA decamer maintains the normal Watson-Crick base pair geometry and causes little distortion in the conformation of its 3'-side. Our results show that in spite of its stable hydrogen bonding, the insertion of a T residue opposite the 3'-T of the cis - syn dimer is inhibited by structural distortion caused by the 3'-T x T base pair. This may explain why the frequency of the 3'-T-->A transversion, which is the major mutation produced by the cis - syn dimer, is only 4%.
About this Structure
Full crystallographic information is available from OCA.
Reference
Solution structure of the DNA decamer duplex containing a 3'-T x T basepair of the cis-syn cyclobutane pyrimidine dimer: implication for the mutagenic property of the cis-syn dimer., Lee JH, Choi YJ, Choi BS, Nucleic Acids Res. 2000 Apr 15;28(8):1794-801. PMID:10734199 Page seeded by OCA on Sat May 3 06:24:20 2008
