3tnf

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (10:10, 1 March 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==LidA from Legionella in complex with active Rab8a==
==LidA from Legionella in complex with active Rab8a==
-
<StructureSection load='3tnf' size='340' side='right' caption='[[3tnf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+
<StructureSection load='3tnf' size='340' side='right'caption='[[3tnf]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._philadelphia_1 Legionella pneumophila subsp. pneumophila str. philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TNF FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[3tnf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TNF FirstGlance]. <br>
-
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene><br>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
-
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3qbt|3qbt]]</td></tr>
+
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MEL, RAB8, RAB8A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), lida, lpg0940 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 Legionella pneumophila subsp. pneumophila str. Philadelphia 1])</td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [https://pdbe.org/3tnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [https://www.ebi.ac.uk/pdbsum/3tnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tnf ProSAT]</span></td></tr>
-
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3tnf RCSB], [http://www.ebi.ac.uk/pdbsum/3tnf PDBsum]</span></td></tr>
+
</table>
-
<table>
+
== Function ==
-
<div style="background-color:#fffaf0;">
+
[https://www.uniprot.org/uniprot/RAB8A_HUMAN RAB8A_HUMAN] May be involved in vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Together with MYO5B and RAB11A participates in epithelial cell polarization.<ref>PMID:20890297</ref> <ref>PMID:21282656</ref>
-
== Publication Abstract from PubMed ==
+
-
The causative agent of Legionnaires disease, Legionella pneumophila, injects several hundred proteins into the cell it infects, many of which interfere with or exploit vesicular transport processes. One of these proteins, LidA, has been described as a Rab effector (i.e., a molecule that interacts preferentially with the GTP-bound form of Rab). We describe here the structure and biochemistry of a complex between the Rab-binding domain of LidA and active Rab8a. LidA displays structural peculiarities in binding to Rab8a, forming a considerably extended interface in comparison to known mammalian Rab effectors, and involving regions of the GTPase that are not seen in other Rab:effector complexes. In keeping with this extended binding interface, which involves four alpha-helices and two pillar-like structures of LidA, the stability of LidA-Rab interactions is dramatically greater than for other such complexes. For Rab1b and Rab8a, these affinities are extraordinarily high, but for the more weakly bound Rab6a, K(d) values of 4 nM for the inactive and 30 pM for the active form were found. Rab1b and Rab8a appear to bind LidA with K(d) values in the low picomolar range, making LidA a Rab supereffector.
+
-
 
+
-
Protein LidA from Legionella is a Rab GTPase supereffector.,Schoebel S, Cichy AL, Goody RS, Itzen A Proc Natl Acad Sci U S A. 2011 Oct 19. PMID:22011575<ref>PMID:22011575</ref>
+
-
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
+
==See Also==
-
</div>
+
*[[Ras-related protein Rab 3D structures|Ras-related protein Rab 3D structures]]
== References ==
== References ==
<references/>
<references/>
Line 21: Line 18:
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Legionella pneumophila subsp. pneumophila str. philadelphia 1]]
+
[[Category: Large Structures]]
-
[[Category: Cichy, A L.]]
+
[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
-
[[Category: Goody, R S.]]
+
[[Category: Cichy AL]]
-
[[Category: Itzen, A.]]
+
[[Category: Goody RS]]
-
[[Category: Schoebel, S.]]
+
[[Category: Itzen A]]
-
[[Category: Drra]]
+
[[Category: Schoebel S]]
-
[[Category: Er]]
+
-
[[Category: Gdp/gtp binding rab-binding]]
+
-
[[Category: Golgi]]
+
-
[[Category: Gtpase]]
+
-
[[Category: Lcv]]
+
-
[[Category: Legionella pneumophila]]
+
-
[[Category: Plasmamembrane]]
+
-
[[Category: Protein transport]]
+
-
[[Category: Rab-effector]]
+
-
[[Category: Rab8a]]
+
-
[[Category: Sidm]]
+
-
[[Category: Vesicle recuitment]]
+
-
[[Category: Vesicular trafficking]]
+
-
[[Category: Vesicular transport]]
+

Current revision

LidA from Legionella in complex with active Rab8a

PDB ID 3tnf

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3tnf"
Personal tools