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| | ==The Crystal Structure of Tat-P(T) (Tp0957)== | | ==The Crystal Structure of Tat-P(T) (Tp0957)== |
| - | <StructureSection load='3u65' size='340' side='right' caption='[[3u65]], [[Resolution|resolution]] 1.40Å' scene=''> | + | <StructureSection load='3u65' size='340' side='right'caption='[[3u65]], [[Resolution|resolution]] 1.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3u65]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Treponema_pallidum_subsp._pallidum Treponema pallidum subsp. pallidum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U65 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3U65 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3u65]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Treponema_pallidum_subsp._pallidum_str._Nichols Treponema pallidum subsp. pallidum str. Nichols]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3U65 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3U65 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3u64|3u64]], [[4di3|4di3]], [[4di4|4di4]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">tp0957, TP_0957 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=161 Treponema pallidum subsp. pallidum])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3u65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u65 OCA], [https://pdbe.org/3u65 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3u65 RCSB], [https://www.ebi.ac.uk/pdbsum/3u65 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3u65 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3u65 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3u65 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3u65 RCSB], [http://www.ebi.ac.uk/pdbsum/3u65 PDBsum]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/O83923_TREPA O83923_TREPA] |
| - | Treponema pallidum, the bacterial agent of syphilis, is predicted to encode one tripartite ATP-independent periplasmic transporter (TRAP-T). TRAP-Ts typically employ a periplasmic substrate-binding protein (SBP) to deliver the cognate ligand to the transmembrane symporter. Herein, we demonstrate that the genes encoding the putative TRAP-T components from T. pallidum, tp0957 (the SBP), and tp0958 (the symporter), are in an operon with an uncharacterized third gene, tp0956. We determined the crystal structure of recombinant Tp0956; the protein is trimeric and perforated by a pore. Part of Tp0956 forms an assembly similar to those of "tetratricopeptide repeat" (TPR) motifs. The crystal structure of recombinant Tp0957 was also determined; like the SBPs of other TRAP-Ts, there are two lobes separated by a cleft. In these other SBPs, the cleft binds a negatively charged ligand. However, the cleft of Tp0957 has a strikingly hydrophobic chemical composition, indicating that its ligand may be substantially different and likely hydrophobic. Analytical ultracentrifugation of the recombinant versions of Tp0956 and Tp0957 established that these proteins associate avidly. This unprecedented interaction was confirmed for the native molecules using in vivo cross-linking experiments. Finally, bioinformatic analyses suggested that this transporter exemplifies a new subfamily of TPATs (TPR-protein-associated TRAP-Ts) that require the action of a TPR-containing accessory protein for the periplasmic transport of a potentially hydrophobic ligand(s).
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| - | Structural, Bioinformatic, and In Vivo Analyses of Two Treponema pallidum Lipoproteins Reveal a Unique TRAP Transporter.,Deka RK, Brautigam CA, Goldberg M, Schuck P, Tomchick DR, Norgard MV J Mol Biol. 2012 Mar 9;416(5):678-96. Epub 2012 Jan 27. PMID:22306465<ref>PMID:22306465</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | == References ==
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| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Treponema pallidum subsp. pallidum]] | + | [[Category: Large Structures]] |
| - | [[Category: Brautigam, C A]] | + | [[Category: Treponema pallidum subsp. pallidum str. Nichols]] |
| - | [[Category: Deka, R K]] | + | [[Category: Brautigam CA]] |
| - | [[Category: Norgard, M V]] | + | [[Category: Deka RK]] |
| - | [[Category: Tomchick, D R]] | + | [[Category: Norgard MV]] |
| - | [[Category: Lipoprotein]]
| + | [[Category: Tomchick DR]] |
| - | [[Category: Protein-protein interaction]]
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| - | [[Category: Syphili]]
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| - | [[Category: Tetratrico peptide repeat]]
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| - | [[Category: Transport protein]]
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| - | [[Category: Treponema pallidum]]
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