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4de9

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==LytR-CPS2A-psr family protein YwtF (TagT) with bound octaprenyl pyrophosphate lipid==
==LytR-CPS2A-psr family protein YwtF (TagT) with bound octaprenyl pyrophosphate lipid==
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<StructureSection load='4de9' size='340' side='right' caption='[[4de9]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
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<StructureSection load='4de9' size='340' side='right'caption='[[4de9]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4de9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DE9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4DE9 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4de9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4DE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4DE9 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=VTP:(2Z,6Z,10Z,14Z,18Z,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>VTP</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.787&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4de8|4de8]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=VTP:(2Z,6Z,10Z,14Z,18Z,22E,26E)-3,7,11,15,19,23,27,31-OCTAMETHYLDOTRIACONTA-2,6,10,14,18,22,26,30-OCTAEN-1-YL+TRIHYDROGEN+DIPHOSPHATE'>VTP</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ywtF, BSU35840 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4de9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4de9 OCA], [https://pdbe.org/4de9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4de9 RCSB], [https://www.ebi.ac.uk/pdbsum/4de9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4de9 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4de9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4de9 OCA], [http://pdbe.org/4de9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4de9 RCSB], [http://www.ebi.ac.uk/pdbsum/4de9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4de9 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/TAGT_BACSU TAGT_BACSU] May catalyze the final step in cell wall teichoic acid biosynthesis, the transfer of the anionic cell wall polymers (APs) from their lipid-linked precursor to the cell wall peptidoglycan (PG).<ref>PMID:21964069</ref>
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Streptococcus pneumoniae protects itself from components of the human immune defense system by a thick polysaccharide capsule, which in most serotypes is covalently attached to the cell wall peptidoglycan. Members of the LytR-Cps2A-Psr (LCP) protein family have recently been implicated in the attachment of anionic polymers to peptidoglycan in Gram-positive bacteria, based on genetic evidence from Bacillus subtilis mutant strains and on the crystal structure of S. pneumoniae Cps2A containing a tightly bound polyprenol (pyro)phosphate lipid. Here, we provide evidence that Cps2A and its two pneumococcal homologs, LytR and Psr, contribute to the maintenance of normal capsule levels and to the retention of the capsular polysaccharide at the cell wall in the capsular type 2 S. pneumoniae strain D39. GFP fusions of all three LCP proteins showed enhanced localization at mid-cell, indicating a role in cell wall growth. Single cps2A or psr mutants produced a reduced amount of capsule. A cps2A lytR double mutant showed greatly impaired growth and cell morphology and lost approximately half of the total capsule material into the culture supernatant. We also present the crystal structure of the B. subtilis LCP protein YwtF and provide crystallographic evidence for the phosphotransferase activity of Cps2A, supporting an enzymatic function in the attachment of capsular polysaccharides to cell wall peptidoglycan.
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Attachment of Capsular Polysaccharide to the Cell Wall in Streptococcus pneumoniae.,Eberhardt A, Hoyland CN, Vollmer D, Bisle S, Cleverley RM, Johnsborg O, Havarstein LS, Lewis RJ, Vollmer W Microb Drug Resist. 2012 Mar 20. PMID:22432711<ref>PMID:22432711</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4de9" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Bacillus globigii migula 1900]]
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[[Category: Bacillus subtilis]]
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[[Category: Bisle, S]]
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[[Category: Large Structures]]
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[[Category: Cleverley, R M]]
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[[Category: Bisle S]]
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[[Category: Eberhardt, A]]
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[[Category: Cleverley RM]]
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[[Category: Havarstein, S]]
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[[Category: Eberhardt A]]
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[[Category: Hoyland, C N]]
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[[Category: Havarstein S]]
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[[Category: Johnsborg, O]]
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[[Category: Hoyland CN]]
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[[Category: Lewis, R J]]
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[[Category: Johnsborg O]]
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[[Category: Vollmer, D]]
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[[Category: Lewis RJ]]
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[[Category: Vollmer, W]]
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[[Category: Vollmer D]]
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[[Category: Membrane protein]]
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[[Category: Vollmer W]]
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[[Category: Possible role in wall techoic acid synthesis]]
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Current revision

LytR-CPS2A-psr family protein YwtF (TagT) with bound octaprenyl pyrophosphate lipid

PDB ID 4de9

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