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4e1s

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==X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7==
==X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7==
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<StructureSection load='4e1s' size='340' side='right' caption='[[4e1s]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
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<StructureSection load='4e1s' size='340' side='right'caption='[[4e1s]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E1S FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4e1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_O157:H7 Escherichia coli O157:H7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4E1S FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.855&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e1t|4e1t]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=OLB:(2S)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLB</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">eae, eaeA, ECs4559, Intimin, L0025, Z5110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [https://pdbe.org/4e1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [https://www.ebi.ac.uk/pdbsum/4e1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4e1s ProSAT]</span></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e1s OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e1s RCSB], [http://www.ebi.ac.uk/pdbsum/4e1s PDBsum]</span></td></tr>
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</table>
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<table>
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== Function ==
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<div style="background-color:#fffaf0;">
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[https://www.uniprot.org/uniprot/EAE_ECO57 EAE_ECO57] Necessary for the production of attaching and effacing lesions on tissue culture cells.
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== Publication Abstract from PubMed ==
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Intimins and invasins are virulence factors produced by pathogenic Gram-negative bacteria. They contain C-terminal extracellular passenger domains that are involved in adhesion to host cells and N-terminal beta domains that are embedded in the outer membrane. Here, we identify the domain boundaries of an E. coli intimin beta domain and use this information to solve its structure and the beta domain structure of a Y. pseudotuberculosis invasin. Both beta domain structures crystallized as monomers and reveal that the previous range of residues assigned to the beta domain also includes a protease-resistant domain that is part of the passenger. Additionally, we identify 146 nonredundant representative members of the intimin/invasin family based on the boundaries of the highly conserved intimin and invasin beta domains. We then use this set of sequences along with our structural data to find and map the evolutionarily constrained residues within the beta domain.
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Crystal Structures of the Outer Membrane Domain of Intimin and Invasin from Enterohemorrhagic E. coli and Enteropathogenic Y. pseudotuberculosis.,Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK Structure. 2012 May 31. PMID:22658748<ref>PMID:22658748</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Escherichia coli]]
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[[Category: Escherichia coli O157:H7]]
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[[Category: Barnard, T J.]]
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[[Category: Large Structures]]
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[[Category: Buchanan, S K.]]
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[[Category: Barnard TJ]]
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[[Category: Cherezov, V.]]
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[[Category: Buchanan SK]]
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[[Category: Dautin, N.]]
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[[Category: Cherezov V]]
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[[Category: Fairman, J W.]]
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[[Category: Dautin N]]
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[[Category: Finkelstein, A.]]
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[[Category: Fairman JW]]
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[[Category: Noinaj, N.]]
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[[Category: Finkelstein A]]
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[[Category: Przytycka, T M.]]
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[[Category: Noinaj N]]
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[[Category: Udho, E.]]
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[[Category: Przytycka TM]]
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[[Category: Wei, L.]]
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[[Category: Udho E]]
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[[Category: Wojtowicz, D.]]
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[[Category: Wei L]]
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[[Category: Adhesin]]
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[[Category: Wojtowicz D]]
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[[Category: Cell adhesion]]
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[[Category: Outer membrane beta barrel]]
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[[Category: Translocated intimin receptor]]
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Current revision

X-ray crystal structure of the transmembrane beta-domain from intimin from EHEC strain O157:H7

PDB ID 4e1s

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