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4eiq

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Chromopyrrolic acid-soaked RebC-10x with bound 7-carboxy-K252c

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 ==Chromopyrrolic acid-soaked RebC-10x with bound 7-carboxy-K252c==
-<StructureSection load='4eiq' size='340' side='right' caption='[[4eiq]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
+<StructureSection load='4eiq' size='340' side='right'caption='[[4eiq]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4eiq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lechevalieria_aerocolonigenes Lechevalieria aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EIQ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4eiq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lentzea_aerocolonigenes Lentzea aerocolonigenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EIQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EIQ FirstGlance]. <br>
-</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCT:(5S)-7-OXO-6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOLE-5-CARBOXYLIC+ACID'>KCT</scene><br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.76&#8491;</td></tr>
-<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2r0g|2r0g]], [[4eip|4eip]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KCT:(5S)-7-OXO-6,7,12,13-TETRAHYDRO-5H-INDOLO[2,3-A]PYRROLO[3,4-C]CARBAZOLE-5-CARBOXYLIC+ACID'>KCT</scene></td></tr>
-<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rbmD, rebC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=68170 Lechevalieria aerocolonigenes])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiq OCA], [https://pdbe.org/4eiq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eiq RCSB], [https://www.ebi.ac.uk/pdbsum/4eiq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eiq ProSAT]</span></td></tr>
-<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eiq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eiq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eiq RCSB], [http://www.ebi.ac.uk/pdbsum/4eiq PDBsum]</span></td></tr>
+</table>
-<table>
+== Function ==
-<div style="background-color:#fffaf0;">
+[https://www.uniprot.org/uniprot/Q8KI25_LENAE Q8KI25_LENAE]
-== Publication Abstract from PubMed ==
-The indolocarbazole biosynthetic enzymes StaC, InkE, RebC, and AtmC mediate the degree of oxidation of chromopyrrolic acid on route to the natural products staurosporine, K252a, rebeccamycin, and AT2433-A1, respectively. Here, we show that StaC and InkE, which mediate a net 4-electron oxidation, bind FAD with a micromolar K(d), whereas RebC and AtmC, which mediate a net 8-electron oxidation, bind FAD with a nanomolar K(d) while displaying the same FAD redox properties. We further create RebC-10x, a RebC protein with ten StaC-like amino acid substitutions outside of previously characterized FAD-binding motifs and the complementary StaC-10x. We find that these mutations mediate both FAD affinity and product specificity, with RebC-10x displaying higher StaC activity than StaC itself. X-ray structures of this StaC catalyst identify the substrate of StaC as 7-carboxy-K252c and suggest a unique mechanism for this FAD-dependent enzyme.
-An Unusual Role for a Mobile Flavin in StaC-like Indolocarbazole Biosynthetic Enzymes.,Goldman PJ, Ryan KS, Hamill MJ, Howard-Jones AR, Walsh CT, Elliott SJ, Drennan CL Chem Biol. 2012 Jul 27;19(7):855-65. PMID:22840773<ref>PMID:22840773</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-== References ==
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Lechevalieria aerocolonigenes]]
+[[Category: Large Structures]]
-[[Category: Drennan, C L.]]
+[[Category: Lentzea aerocolonigenes]]
-[[Category: Elliott, S J.]]
+[[Category: Drennan CL]]
-[[Category: Goldman, P J.]]
+[[Category: Elliott SJ]]
-[[Category: Hamill, M J.]]
+[[Category: Goldman PJ]]
-[[Category: Howard-Jones, A R.]]
+[[Category: Hamill MJ]]
-[[Category: Ryan, K S.]]
+[[Category: Howard-Jones AR]]
-[[Category: Walsh, C T.]]
+[[Category: Ryan KS]]
-[[Category: 7-carboxy-k252c]]
+[[Category: Walsh CT]]
-[[Category: Flavin adenine dinucleotide]]
-[[Category: Indolocarbazole]]
-[[Category: Monooxygenase]]
-[[Category: Oxidoreductase-oxidoreductase inhibitor complex]]

4eiq

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Chromopyrrolic acid-soaked RebC-10x with bound 7-carboxy-K252c

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