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4ets

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==Crystal structure of Campylobacter jejuni ferric uptake regulator==
==Crystal structure of Campylobacter jejuni ferric uptake regulator==
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<StructureSection load='4ets' size='340' side='right' caption='[[4ets]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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<StructureSection load='4ets' size='340' side='right'caption='[[4ets]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ets]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ETS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ETS FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ets]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni Campylobacter jejuni subsp. jejuni]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ETS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ETS FirstGlance]. <br>
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</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fur, Cj0400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=32022 Campylobacter jejuni subsp. jejuni])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ets OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4ets RCSB], [http://www.ebi.ac.uk/pdbsum/4ets PDBsum]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ets FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ets OCA], [https://pdbe.org/4ets PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ets RCSB], [https://www.ebi.ac.uk/pdbsum/4ets PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ets ProSAT]</span></td></tr>
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<table>
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</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/FUR_CAMJE FUR_CAMJE] Acts as a global negative controlling element, employing Fe(2+) as a cofactor to bind the operator of the repressed genes.
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The full regulatory potential of the ferric uptake regulator (Fur) family of proteins remains undefined despite over 20 years of study. We report herein an integrated approach that combines both genome-wide technologies and structural studies to define the role of Fur in Campylobacter jejuni (Cj). CjFur ChIP-chip assays identified 95 genomic loci bound by CjFur associated with functions as diverse as iron acquisition, flagellar biogenesis, and non-iron ion transport. Comparative analysis with transcriptomic data revealed that CjFur regulation extends beyond solely repression and also includes both gene activation and iron-independent regulation. Computational analysis revealed the presence of an elongated holo-Fur repression motif along with a divergent holo-Fur activation motif. This diversity of CjFur DNA-binding elements is supported by the crystal structure of CjFur, which revealed a unique conformation of its DNA-binding domain and the absence of metal in the regulatory site. Strikingly, our results indicate that the apo-CjFur structure retains the canonical V-shaped dimer reminiscent of previously characterized holo-Fur proteins enabling DNA interaction. This conformation stems from a structurally unique hinge domain that is poised to further contribute to CjFur's regulatory functions by modulating the orientation of the DNA-binding domain upon binding of iron. The unique features of the CjFur crystal structure rationalize the binding sequence diversity that was uncovered during ChIP-chip analysis and defines apo-Fur regulation.
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Structure and regulon of Campylobacter jejuni ferric uptake regulator Fur define apo-Fur regulation.,Butcher J, Sarvan S, Brunzelle JS, Couture JF, Stintzi A Proc Natl Acad Sci U S A. 2012 Jun 4. PMID:22665794<ref>PMID:22665794</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Campylobacter jejuni subsp. jejuni]]
[[Category: Campylobacter jejuni subsp. jejuni]]
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[[Category: Brunzelle, J B.]]
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[[Category: Large Structures]]
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[[Category: Couture, J F.]]
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[[Category: Brunzelle JB]]
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[[Category: Sarvan, S.]]
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[[Category: Couture J-F]]
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[[Category: Metal binding protein]]
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[[Category: Sarvan S]]
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[[Category: Transcription factor]]
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Current revision

Crystal structure of Campylobacter jejuni ferric uptake regulator

PDB ID 4ets

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