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4etx

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==Crystal Structure of PelD 158-CT from Pseudomonas aeruginosa PAO1==
==Crystal Structure of PelD 158-CT from Pseudomonas aeruginosa PAO1==
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<StructureSection load='4etx' size='340' side='right' caption='[[4etx]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
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<StructureSection load='4etx' size='340' side='right'caption='[[4etx]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4etx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ETX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ETX FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4etx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ETX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ETX FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4etz|4etz]], [[4eu0|4eu0]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PA3061, pelD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4etx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4etx OCA], [https://pdbe.org/4etx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4etx RCSB], [https://www.ebi.ac.uk/pdbsum/4etx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4etx ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4etx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4etx OCA], [http://pdbe.org/4etx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4etx RCSB], [http://www.ebi.ac.uk/pdbsum/4etx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4etx ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q9HZE7_PSEAE Q9HZE7_PSEAE]
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The second messenger bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) plays a vital role in the global regulation in bacteria. Here, we describe structural and biochemical characterization of a novel c-di-GMP effector PelD that is critical to the formation of pellicles by Pseudomonas aeruginosa. We present high-resolution structures of a cytosolic fragment of PelD in apo form and its complex with c-di-GMP. The structure contains a bi-domain architecture composed of a GAF domain (commonly found in cyclic nucleotide receptors) and a GGDEF domain (found in c-di-GMP synthesizing enzymes), with the latter binding to one molecule of c-di-GMP. The GGDEF domain has a degenerate active site but a conserved allosteric site (I-site), which we show binds c-di-GMP with a K(d) of 0.5 mum. We identified a series of residues that are crucial for c-di-GMP binding, and confirmed the roles of these residues through biochemical characterization of site-specific variants. The structures of PelD represent a novel class of c-di-GMP effector and expand the knowledge of scaffolds that mediate c-di-GMP recognition.
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Structures of the PelD Cyclic Diguanylate Effector Involved in Pellicle Formation in Pseudomonas aeruginosa PAO1.,Li Z, Chen JH, Hao Y, Nair SK J Biol Chem. 2012 Aug 31;287(36):30191-204. Epub 2012 Jul 17. PMID:22810222<ref>PMID:22810222</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4etx" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Pseae]]
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[[Category: Large Structures]]
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[[Category: Chen, J]]
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[[Category: Pseudomonas aeruginosa PAO1]]
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[[Category: Li, Z]]
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[[Category: Chen J]]
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[[Category: Nair, S K]]
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[[Category: Li Z]]
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[[Category: C-di-gmp]]
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[[Category: Nair SK]]
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[[Category: Signaling protein]]
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Current revision

Crystal Structure of PelD 158-CT from Pseudomonas aeruginosa PAO1

PDB ID 4etx

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