4eyy

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==Crystal Structure of the IcmR-IcmQ complex from Legionella pneumophila==
==Crystal Structure of the IcmR-IcmQ complex from Legionella pneumophila==
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<StructureSection load='4eyy' size='340' side='right' caption='[[4eyy]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
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<StructureSection load='4eyy' size='340' side='right'caption='[[4eyy]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4eyy]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Legpc Legpc] and [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EYY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EYY FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4eyy]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_str._Corby Legionella pneumophila str. Corby] and [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EYY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EYY FirstGlance]. <br>
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</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3fxd|3fxd]], [[3fxe|3fxe]]</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">icmR, lpg0443 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH]), icmQ, LPC_2899 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=400673 LEGPC])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eyy OCA], [https://pdbe.org/4eyy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eyy RCSB], [https://www.ebi.ac.uk/pdbsum/4eyy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eyy ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eyy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eyy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eyy RCSB], [http://www.ebi.ac.uk/pdbsum/4eyy PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q5ZYC9_LEGPH Q5ZYC9_LEGPH]
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A Type 4b secretion system (T4bSS) is required for Legionella growth in alveolar macrophages. IcmQ associates with IcmR, binds to membranes, and has a critical role in the T4bSS. We have now solved a crystal structure of IcmR-IcmQ to further our understanding of this complex. This structure revealed an amphipathic four-helix bundle, formed by IcmR and the N-terminal domain of IcmQ, which is linked to a novel C-terminal domain of IcmQ (Qc) by a linker helix. The Qc domain has structural homology with ADP ribosyltransferase domains in certain bacterial toxins and binds NAD(+) with a dissociation constant in the physiological range. Structural homology and molecular dynamics were used to identify an extended NAD(+) binding site on Qc, and the resulting model was tested by mutagenesis and binding assays. Based on the data, we suggest that IcmR-IcmQ binds to membranes, where it may interact with, or perhaps modify, a protein in the T4bSS when NAD(+) is bound.
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IcmQ in the Type 4b secretion system contains an NAD+ binding domain.,Farelli JD, Gumbart JC, Akey IV, Hempstead A, Amyot W, Head JF, McKnight CJ, Isberg RR, Akey CW Structure. 2013 Aug 6;21(8):1361-73. doi: 10.1016/j.str.2013.05.017. Epub 2013, Jul 11. PMID:23850453<ref>PMID:23850453</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Legpc]]
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[[Category: Large Structures]]
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[[Category: Legph]]
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[[Category: Legionella pneumophila str. Corby]]
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[[Category: Akey, C W]]
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[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
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[[Category: Akey, I V]]
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[[Category: Akey CW]]
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[[Category: Amyot, W]]
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[[Category: Akey IV]]
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[[Category: Farelli, J D]]
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[[Category: Amyot W]]
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[[Category: Gumbart, J]]
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[[Category: Farelli JD]]
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[[Category: Head, J F]]
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[[Category: Gumbart J]]
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[[Category: Hempstead, A D]]
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[[Category: Head JF]]
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[[Category: Isberg, R R]]
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[[Category: Hempstead AD]]
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[[Category: McKnight, C J]]
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[[Category: Isberg RR]]
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[[Category: Adprt-like fold]]
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[[Category: McKnight CJ]]
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[[Category: Nad-binding domain]]
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[[Category: Protein binding]]
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[[Category: Protein heterodimer]]
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Current revision

Crystal Structure of the IcmR-IcmQ complex from Legionella pneumophila

PDB ID 4eyy

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