This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4eza

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:07, 1 March 2024) (edit) (undo)
 
(3 intermediate revisions not shown.)
Line 1: Line 1:
 +
==Crystal structure of the atypical phosphoinositide (aPI) binding domain of IQGAP2==
==Crystal structure of the atypical phosphoinositide (aPI) binding domain of IQGAP2==
-
<StructureSection load='4eza' size='340' side='right' caption='[[4eza]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+
<StructureSection load='4eza' size='340' side='right'caption='[[4eza]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4eza]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EZA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4EZA FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4eza]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4EZA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4EZA FirstGlance]. <br>
-
</td></tr><tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">IQGAP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4eza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eza OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4eza RCSB], [http://www.ebi.ac.uk/pdbsum/4eza PDBsum]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4eza FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4eza OCA], [https://pdbe.org/4eza PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4eza RCSB], [https://www.ebi.ac.uk/pdbsum/4eza PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4eza ProSAT]</span></td></tr>
-
<table>
+
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/IQGA2_HUMAN IQGA2_HUMAN] Binds to activated CDC42 and RAC1 but does not seem to stimulate their GTPase activity. Associates with calmodulin.
-
Class I phosphoinositide (PI) 3-kinases act through effector proteins whose 3-PI selectivity is mediated by a limited repertoire of structurally defined, lipid recognition domains. We describe here the lipid preferences and crystal structure of a new class of PI binding module exemplified by select IQGAPs (IQ motif containing GTPase activating proteins) known to co-ordinate cellular signalling events and cytoskeletal dynamics. This module is defined by a C-terminal 105-107 amino acid region of which that from IQGAP1 and 2, but not IQGAP3, binds preferentially to phosphatidylinositol 3,4,5-trisphosphate (PtdInsP(3)). The binding affinity for PtdInsP(3), together with other, secondary target-recognition characteristics, are comparable to those of the pleckstrin homology (PH) domain of cytohesin-3 (general receptor for phosphoinositides 1), an established PtdInsP(3) effector protein. Importantly, the IQGAP1 C-terminal domain and the cytohesin-3 PH domain, each tagged with enhanced green fluorescent protein, both re-localized from the cytosol to the cell periphery following the activation of PI 3-kinase in Swiss 3T3 fibroblasts, consistent with their common, selective recognition of endogenous 3-PI(s). The crystal structure of the C-terminal IQGAP2 PI binding module reveals unexpected topological similarity to an integral fold of C2 domains, including a putative basic binding pocket. We propose that this module integrates select IQGAP proteins with PI 3-kinase signalling and constitutes a novel, atypical phosphoinositide (aPI) binding domain that may represent the first of a larger group, each perhaps structurally unique but collectively dissimilar from the known PI recognition modules.
+
-
 
+
-
IQGAP proteins reveal an atypical phosphoinositide (aPI) binding domain with a pseudo C2 domain fold.,Dixon MJ, Gray A, Schenning M, Agacan M, Tempel W, Tong Y, Nedyalkova L, Park HW, Leslie NR, van Aalten DM, Downes CP, Batty IH J Biol Chem. 2012 Apr 5. PMID:22493426<ref>PMID:22493426</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
-
[[Category: Aalten, D M.F Van.]]
+
[[Category: Large Structures]]
-
[[Category: Agacan, M.]]
+
[[Category: Agacan M]]
-
[[Category: Arrowsmith, C H.]]
+
[[Category: Arrowsmith CH]]
-
[[Category: Batty, I H.]]
+
[[Category: Batty IH]]
-
[[Category: Bochkarev, A.]]
+
[[Category: Bochkarev A]]
-
[[Category: Bountra, C.]]
+
[[Category: Bountra C]]
-
[[Category: Crombet, L.]]
+
[[Category: Crombet L]]
-
[[Category: Dixon, M J.]]
+
[[Category: Dixon MJ]]
-
[[Category: Downes, C P.]]
+
[[Category: Downes CP]]
-
[[Category: Edwards, A M.]]
+
[[Category: Edwards AM]]
-
[[Category: Gray, A.]]
+
[[Category: Gray A]]
-
[[Category: Leslie, N R.]]
+
[[Category: Leslie NR]]
-
[[Category: Nedyalkova, L.]]
+
[[Category: Nedyalkova L]]
-
[[Category: Park, H.]]
+
[[Category: Park H]]
-
[[Category: SGC, Structural Genomics Consortium.]]
+
[[Category: Schenning M]]
-
[[Category: Schenning, M.]]
+
[[Category: Tempel W]]
-
[[Category: Tempel, W.]]
+
[[Category: Tong Y]]
-
[[Category: Tong, Y.]]
+
[[Category: Van Aalten DMF]]
-
[[Category: Weigelt, J.]]
+
[[Category: Weigelt J]]
-
[[Category: Zhong, N.]]
+
[[Category: Zhong N]]
-
[[Category: Gap]]
+
-
[[Category: Sgc]]
+
-
[[Category: Signaling protein]]
+
-
[[Category: Structural genomics consortium]]
+

Current revision

Crystal structure of the atypical phosphoinositide (aPI) binding domain of IQGAP2

PDB ID 4eza

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4eza"
Personal tools