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4f87

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==X-ray Crystal Structure of PlyCB==
==X-ray Crystal Structure of PlyCB==
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<StructureSection load='4f87' size='340' side='right' caption='[[4f87]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
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<StructureSection load='4f87' size='340' side='right'caption='[[4f87]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4f87]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptococcus_phage_c1 Streptococcus phage c1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F87 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4F87 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4f87]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_virus_C1 Streptococcus virus C1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F87 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F87 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4f88|4f88]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">orf9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=230871 Streptococcus phage C1])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f87 OCA], [https://pdbe.org/4f87 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f87 RCSB], [https://www.ebi.ac.uk/pdbsum/4f87 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f87 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4f87 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f87 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4f87 RCSB], [http://www.ebi.ac.uk/pdbsum/4f87 PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q7Y3F3_9CAUD Q7Y3F3_9CAUD]
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Bacteriophages deploy lysins that degrade the bacterial cell wall and facilitate virus egress from the host. When applied exogenously, these enzymes destroy susceptible microbes and, accordingly, have potential as therapeutic agents. The most potent lysin identified to date is PlyC, an enzyme assembled from two components (PlyCA and PlyCB) that is specific for streptococcal species. Here the structure of the PlyC holoenzyme reveals that a single PlyCA moiety is tethered to a ring-shaped assembly of eight PlyCB molecules. Structure-guided mutagenesis reveals that the bacterial cell wall binding is achieved through a cleft on PlyCB. Unexpectedly, our structural data reveal that PlyCA contains a glycoside hydrolase domain in addition to the previously recognized cysteine, histidine-dependent amidohydrolases/peptidases catalytic domain. The presence of eight cell wall-binding domains together with two catalytic domains may explain the extraordinary potency of the PlyC holoenyzme toward target bacteria.
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X-ray crystal structure of the streptococcal specific phage lysin PlyC.,McGowan S, Buckle AM, Mitchell MS, Hoopes JT, Gallagher DT, Heselpoth RD, Shen Y, Reboul CF, Law RH, Fischetti VA, Whisstock JC, Nelson DC Proc Natl Acad Sci U S A. 2012 Jul 17. PMID:22807482<ref>PMID:22807482</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Streptococcus phage c1]]
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[[Category: Large Structures]]
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[[Category: Buckle, A M]]
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[[Category: Streptococcus virus C1]]
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[[Category: Fischetti, V A]]
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[[Category: Buckle AM]]
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[[Category: McGowan, S]]
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[[Category: Fischetti VA]]
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[[Category: Nelson, D C]]
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[[Category: McGowan S]]
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[[Category: Whisstock, J C]]
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[[Category: Nelson DC]]
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[[Category: Antimicrobial protein]]
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[[Category: Whisstock JC]]
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[[Category: Bacteriophage]]
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[[Category: Lysin]]
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[[Category: Viral protein]]
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Current revision

X-ray Crystal Structure of PlyCB

PDB ID 4f87

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