4gkv

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==Structure of Escherichia coli AdhP (ethanol-inducible dehydrogenase) with bound NAD==
==Structure of Escherichia coli AdhP (ethanol-inducible dehydrogenase) with bound NAD==
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<StructureSection load='4gkv' size='340' side='right' caption='[[4gkv]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
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<StructureSection load='4gkv' size='340' side='right'caption='[[4gkv]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4gkv]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [http://en.wikipedia.org/wiki/Escherichia_coli_k-12 Escherichia coli k-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GKV FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4gkv]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GKV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4GKV FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.008&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">adhP, yddN, b1478, JW1474 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 Escherichia coli K-12])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alcohol_dehydrogenase Alcohol dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.1 1.1.1.1] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4gkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gkv OCA], [https://pdbe.org/4gkv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4gkv RCSB], [https://www.ebi.ac.uk/pdbsum/4gkv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4gkv ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gkv OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gkv RCSB], [http://www.ebi.ac.uk/pdbsum/4gkv PDBsum]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/ADHP_ECOLI ADHP_ECOLI] Preferred specificity is towards 1-propanol.
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The crystal structure of AdhP, a recombinantly expressed alcohol dehydrogenase from Escherichia coli K-12 (substrain MG1655), was determined to 2.01 A resolution. The structure, which was solved using molecular replacement, also included the structural and catalytic zinc ions and the cofactor nicotinamide adenine dinucleotide (NAD). The crystals belonged to space group P21, with unit-cell parameters a = 68.18, b = 118.92, c = 97.87 A, beta = 106.41 degrees . The final R factor and Rfree were 0.138 and 0.184, respectively. The structure of the active site of AdhP suggested a number of residues that may participate in a proton relay, and the overall structure of AdhP, including the coordination to structural and active-site zinc ions, is similar to those of other tetrameric alcohol dehydrogenase enzymes.
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Structure of Escherichia coli AdhP (ethanol-inducible dehydrogenase) with bound NAD.,Thomas LM, Harper AR, Miner WA, Ajufo HO, Branscum KM, Kao L, Sims PA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul 1;69(Pt 7):730-2. doi:, 10.1107/S1744309113015170. Epub 2013 Jun 27. PMID:23832197<ref>PMID:23832197</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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==See Also==
==See Also==
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*[[Alcohol dehydrogenase|Alcohol dehydrogenase]]
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*[[Alcohol dehydrogenase 3D structures|Alcohol dehydrogenase 3D structures]]
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Alcohol dehydrogenase]]
 
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
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[[Category: Escherichia coli k-12]]
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[[Category: Escherichia coli K-12]]
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[[Category: Ajufo, H O]]
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[[Category: Large Structures]]
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[[Category: Branscrum, K M]]
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[[Category: Ajufo HO]]
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[[Category: Harper, A R]]
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[[Category: Branscrum KM]]
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[[Category: Kao, L]]
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[[Category: Harper AR]]
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[[Category: Miner, W A]]
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[[Category: Kao L]]
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[[Category: Sims, P A]]
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[[Category: Miner WA]]
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[[Category: Thomas, L M]]
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[[Category: Sims PA]]
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[[Category: Oxidoreductase]]
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[[Category: Thomas LM]]

Current revision

Structure of Escherichia coli AdhP (ethanol-inducible dehydrogenase) with bound NAD

PDB ID 4gkv

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