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4hcp

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{{STRUCTURE_4hcp| PDB=4hcp | SCENE= }}
 
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===crystal structure of Burkholderia pseudomallei effector protein chbp in complex with nedd8===
 
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{{ABSTRACT_PUBMED_23175788}}
 
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==Function==
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==crystal structure of Burkholderia pseudomallei effector protein chbp in complex with nedd8==
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[[http://www.uniprot.org/uniprot/NEDD8_HUMAN NEDD8_HUMAN]] Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.<ref>PMID:10318914</ref> <ref>PMID:10597293</ref> <ref>PMID:11953428</ref>
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<StructureSection load='4hcp' size='340' side='right'caption='[[4hcp]], [[Resolution|resolution]] 2.52&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[4hcp]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei_K96243 Burkholderia pseudomallei K96243] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HCP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HCP FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.52&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hcp OCA], [https://pdbe.org/4hcp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hcp RCSB], [https://www.ebi.ac.uk/pdbsum/4hcp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hcp ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CIF_BURPS CIF_BURPS] Protein-glutamine deamidase effector that inhibits the host cell cycle and other key cellular processes such as the actin network and programmed-cell death (PubMed:19308257, PubMed:20688984, PubMed:19225106). Acts by mediating the side chain deamidation of 'Gln-40' of host NEDD8, converting it to glutamate, thereby abolishing the activity of cullin-RING-based E3 ubiquitin-protein ligase complexes (CRL complexes) (PubMed:20688984, PubMed:21903097). Inactivation of CRL complexes prevents ubiquitination and subsequent degradation of the cyclin-dependent kinase inhibitors CDKN1A/p21 and CDKN1B/p27, leading to G1 and G2 cell cycle arrests in host cells (PubMed:19308257). Deamidation of 'Gln-40' of host NEDD8 also triggers macrophage-specific programmed cell death (PubMed:23175788). Also able to catalyze deamidation of 'Gln-40' of host ubiquitin in vitro; however, NEDD8 constitutes the preferred substrate in vivo (PubMed:20688984). Also regulates the host NF-kappa-B signaling via activation of MAPK/ERK cascade: activation of host MAPK/ERK cascade is independent of CRL complexes inhibition, suggesting that Cif has other host protein targets than NEDD8 (PubMed:28166272, PubMed:29848489).<ref>PMID:19225106</ref> <ref>PMID:19308257</ref> <ref>PMID:20688984</ref> <ref>PMID:21903097</ref> <ref>PMID:23175788</ref> <ref>PMID:28166272</ref> <ref>PMID:29848489</ref>
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==About this Structure==
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==See Also==
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[[4hcp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Burps Burps] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HCP OCA].
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*[[NEDD8|NEDD8]]
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== References ==
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==Reference==
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<references/>
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<ref group="xtra">PMID:023175788</ref><references group="xtra"/><references/>
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__TOC__
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[[Category: Burps]]
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</StructureSection>
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[[Category: Human]]
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[[Category: Burkholderia pseudomallei K96243]]
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[[Category: Shao, F.]]
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[[Category: Homo sapiens]]
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[[Category: Yao, Q.]]
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[[Category: Large Structures]]
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[[Category: Alpha/beta/alpha fold]]
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[[Category: Shao F]]
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[[Category: Bacterial cytosol]]
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[[Category: Yao Q]]
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[[Category: Deamidase]]
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[[Category: Deamidation]]
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[[Category: Nedd8/ubiquitin]]
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[[Category: Protein binding]]
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Current revision

crystal structure of Burkholderia pseudomallei effector protein chbp in complex with nedd8

PDB ID 4hcp

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