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4hh4

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==Structure of the CcbJ Methyltransferase from Streptomyces caelestis==
==Structure of the CcbJ Methyltransferase from Streptomyces caelestis==
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<StructureSection load='4hh4' size='340' side='right' caption='[[4hh4]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
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<StructureSection load='4hh4' size='340' side='right'caption='[[4hh4]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4hh4]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1688 As 4.1688]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HH4 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4hh4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_caelestis Streptomyces caelestis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HH4 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4hgy|4hgy]], [[4hgz|4hgz]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ccbJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=36816 AS 4.1688])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hh4 OCA], [https://pdbe.org/4hh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hh4 RCSB], [https://www.ebi.ac.uk/pdbsum/4hh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hh4 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hh4 OCA], [http://pdbe.org/4hh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hh4 RCSB], [http://www.ebi.ac.uk/pdbsum/4hh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hh4 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/E9JES0_9ACTN E9JES0_9ACTN]
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The S-adenosyl-L-methionine (SAM)-dependent methyltransferase CcbJ from Streptomyces caelestis catalyzes one of the final steps in the biosynthesis of the antibiotic celesticetin, methylation of the N atom of its proline moiety, which greatly enhances the activity of the antibiotic. Since several celesticetin variants exist, this enzyme may be able to act on a variety of substrates. The structures of CcbJ determined by MAD phasing at 3.0 A resolution, its native form at 2.7 A resolution and its complex with S-adenosyl-L-homocysteine (SAH) at 2.9 A resolution are reported here. Based on these structures, three point mutants, Y9F, Y17F and F117G, were prepared in order to study its behaviour as well as docking simulations of both CcbJ-SAM-substrate and CcbJ-SAH-product complexes. The structures show that CcbJ is a class I SAM-dependent methyltransferase with a wide active site, thereby suggesting that it may accommodate a number of different substrates. The mutation results show that the Y9F and F117G mutants are almost non-functional, while the Y17F mutant has almost half of the wild-type activity. In combination with the docking studies, these results suggest that Tyr9 and Phe117 are likely to help to position the substrate for the methyl-transfer reaction and that Tyr9 may also facilitate the reaction by removing an H(+) ion. Tyr17, on the other hand, seems to operate by helping to stabilize the SAM cofactor.
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Structure and possible mechanism of the CcbJ methyltransferase from Streptomyces caelestis.,Bauer J, Ondrovicova G, Najmanova L, Pevala V, Kamenik Z, Kostan J, Janata J, Kutejova E Acta Crystallogr D Biol Crystallogr. 2014 Apr 1;70(Pt 4):943-57. doi:, 10.1107/S139900471303397X. Epub 2014 Mar 19. PMID:24699640<ref>PMID:24699640</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4hh4" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: As 4 1688]]
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[[Category: Large Structures]]
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[[Category: Bauer, J A]]
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[[Category: Streptomyces caelestis]]
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[[Category: Janata, J]]
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[[Category: Bauer JA]]
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[[Category: Kutejova, E]]
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[[Category: Janata J]]
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[[Category: Ondrovicova, G]]
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[[Category: Kutejova E]]
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[[Category: Methyltransferase]]
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[[Category: Ondrovicova G]]
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[[Category: S-adenosyl-l-methionine-dependent methyltransferase]]
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[[Category: Transferase]]
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Current revision

Structure of the CcbJ Methyltransferase from Streptomyces caelestis

PDB ID 4hh4

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