This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


4hic

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (11:41, 1 March 2024) (edit) (undo)
 
(One intermediate revision not shown.)
Line 1: Line 1:
==Crystal structure of the potential transfer protein TraK from Gram-positive conjugative plasmid pIP501==
==Crystal structure of the potential transfer protein TraK from Gram-positive conjugative plasmid pIP501==
-
<StructureSection load='4hic' size='340' side='right' caption='[[4hic]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
+
<StructureSection load='4hic' size='340' side='right'caption='[[4hic]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
-
<table><tr><td colspan='2'>[[4hic]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"enterococcus_proteiformis"_thiercelin_and_jouhaud_1903 "enterococcus proteiformis" thiercelin and jouhaud 1903]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4HIC FirstGlance]. <br>
+
<table><tr><td colspan='2'>[[4hic]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_faecalis Enterococcus faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4HIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4HIC FirstGlance]. <br>
-
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ORF11 from conjugative plasmid pIP501 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1351 "Enterococcus proteiformis" Thiercelin and Jouhaud 1903])</td></tr>
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.001&#8491;</td></tr>
-
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4hic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hic OCA], [http://pdbe.org/4hic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4hic RCSB], [http://www.ebi.ac.uk/pdbsum/4hic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4hic ProSAT]</span></td></tr>
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4hic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4hic OCA], [https://pdbe.org/4hic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4hic RCSB], [https://www.ebi.ac.uk/pdbsum/4hic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4hic ProSAT]</span></td></tr>
</table>
</table>
-
<div style="background-color:#fffaf0;">
+
== Function ==
-
== Publication Abstract from PubMed ==
+
[https://www.uniprot.org/uniprot/Q8L1C9_ENTFL Q8L1C9_ENTFL]
-
Conjugative plasmid transfer presents a serious threat to human health as the most important means of spreading antibiotic resistance and virulence genes among bacteria. The required direct cell-cell contact is established by a multi-protein complex, the conjugative type IV secretion system (T4SS). The conjugative core complex spans the cellular envelope and serves as a channel for macromolecular secretion. T4SSs of Gram-negative (G-) origin have been studied in great detail. In contrast, T4SSs of Gram-positive (G+) bacteria have only received little attention thus far, despite the medical relevance of numerous G+ pathogens (e.g. enterococci, staphylococci and streptococci). This study provides structural information on the type IV secretion (T4S) protein TraK of the G+ broad host range Enterococcus conjugative plasmid pIP501. The crystal structure of the N-terminally truncated construct TraKDelta was determined to 3.0 A resolution and exhibits a novel fold. Immunolocalization demonstrated that the protein localizes to the cell wall facing towards the cell exterior, but does not exhibit surface accessibility. Circular dichroism, dynamic light scattering and size-exclusion chromatography confirmed the protein to be a monomer. With the exception of proteins from closely related T4SSs, no significant sequence or structural relatives were found. This observation marks the protein as a very exclusive, specialized member of the pIP501 T4SS.
+
-
 
+
-
The type IV secretion protein TraK from the Enterococcus conjugative plasmid pIP501 exhibits a novel fold.,Goessweiner-Mohr N, Fercher C, Arends K, Birner-Gruenberger R, Laverde-Gomez D, Huebner J, Grohmann E, Keller W Acta Crystallogr D Biol Crystallogr. 2014 Apr;70(Pt 4):1124-35. doi:, 10.1107/S1399004714001606. Epub 2014 Mar 21. PMID:24699656<ref>PMID:24699656</ref>
+
-
 
+
-
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+
-
</div>
+
-
<div class="pdbe-citations 4hic" style="background-color:#fffaf0;"></div>
+
-
== References ==
+
-
<references/>
+
__TOC__
__TOC__
</StructureSection>
</StructureSection>
-
[[Category: Enterococcus proteiformis thiercelin and jouhaud 1903]]
+
[[Category: Enterococcus faecalis]]
-
[[Category: Goessweiner-Mohr, N]]
+
[[Category: Large Structures]]
-
[[Category: Keller, W]]
+
[[Category: Goessweiner-Mohr N]]
-
[[Category: Anti-parallel beta-sheet]]
+
[[Category: Keller W]]
-
[[Category: Gram-positive]]
+
-
[[Category: Pip501]]
+
-
[[Category: Type-iv secretion]]
+
-
[[Category: Unknown function]]
+

Current revision

Crystal structure of the potential transfer protein TraK from Gram-positive conjugative plasmid pIP501

PDB ID 4hic

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4hic"
Personal tools